| Objective: The purpose of this study is to do systematic studies on anti tumor activity of Melodinus khasianus, then screen out chemical components with anti tu mor activity. This study provides technical supports for the quality control and utili zation of Melodinus khasianus, and lay a theoretical foundation for the development of new anti tumor drug.Method: According to the object of this research, sort out related literatures o n Melodinus khasianus, and make summaries of its chemical composition, pharmaco logical effect and sources. Separates chemical components from Melodinus khasianus by using silica gel column chromatography, reversed-phase C18 column chromatogr aphy, high-speed countercurrent chromatography and high performance liquid chroma tography, and the monomer compounds are identified by 1H-NMR,13C-NMR, HMBC,HMQC, IR, UV, HR-ESI, HR-ESI-MS combined with data from related literatures.Results: 18 monomer compounds are isolated from Melodinus khasianus by us e of different column chromatographies, like HPLC and HSCCC, they are Melodini ne T 1、16β,21β-Epoxy-vincadine 2、pleiocarpamine 4、Melodinine G 5、+)epi-16 d khydrol4-15 vincamine 6、Melodinine P 7、19-Epimeloscandonine 9、meloscandonine 10、16Hydroxy-19S-vindolinine 11、Melodinine W 12、l5α-Hydroxy-14,15-dihydrovi ndolinine 13、Melodinine V 14、16fl-Hydroxy-19R-vindolinine 15、scandine 16、Me lodinine N 17、tabersonine 18. Among them, Melodinine W 12, Melodinine V 14 a re new compounds, Activity test results showed that Melodinine N 17 have strong anti tumor activity, these tests provide basis for the development of new drugs agai nst tumor.Conclusion: The carrying out and achievements of this research further determi ne that some monomer compounds from ethanol fraction of Melodinus khasianus ha ve anti tumor activity, and provides certain technical supports for further developme nts and applications of Herpetospermum caudigerum Wall resources. |
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