| Objective: The formation mechanism of interstitial lung disease is complicated. It is one of refractory diseases in modern medicine, bringing great detress to people. At present, western medicine in the treatment of interstitial lung disease is unsatisfactory. In recent years, there are more prominent advantages of traditional Chinese medicine in the treatment of interstitial lung disease in hand. This topic on the interstitial lung disease is the most important of phlegm and blood stasis obstruction syndrome patients with clinical observation and explore the Huoxue Tongluo prescription in the treatment of phlegm stasis Bizu interstitial lung disease of the treatment effect. In order to further clarify the specific ways of traditional Chinese medicine in the treatment of diseases, we design an animal experimental study. To provide theoretical support for the application of drugs.Methods: The clinical observation part: We put the phlegm and blood stasis type of idiopathic interstitial lung disease among 34 patients divided into 2 groups. Western medicine treatment group were treated by hormone combined with N-acetylcysteine. The treatment group with combination of TCM and Western Medicine were treated by hormone combined with N-acetylcysteine while treated by the prescription of "Xian Luo lung yin". To observe the application of drug treatment in patients after CT, lung function, symptoms of change. The data using SPSS17.0 software for statistical analysis of experimental data. The animal experimental part: We kept 80 clean grade healthy male Wistar rats in IVC cage. Room temperature(24 DEG to 26 DEG), maintain humidity. Bedding, food and water sterilization. In the course of the experiment does not limit the fresh water. Adaptive feeding after 3D, rats were randomly divided into 8 groups:Blank group(group A), interstitial lung disease model group(group B), high concentrations of the centipede group(Group U), concentration of centipede group(Group V), low concentration of centipede group(W Group), pirfenidone group(x group), pirfenidone with centipede group(Y group), Jodison+N-acetylcysteine+azathioprine group(group Z).Adaptive feeding rats 3 days after modeling. B, X, Z, U, V, W, Y group rats by intratracheal perfusion into the liquor bleomycin(5mg / kg), group a rats by intratracheal perfusion in equal volume of sterile saline.After given drugs to fill the stomach every day, the rats in each group were sacrificed at twenty-eighth days. Under the light microscope to observe the pathological changes of lung tissue of rats in each group. The histological change. Content by chemical fluorescence was detected in the lung tissues of rats with SOD, MDA. Expression of ELISA was detected in rat aortic IL-8, serum type I collagen. The expression level of VEGF-mRNA, TGF- beta 1 in lung tissue was measured by using PCR method. The expression level was observed by immunohistochemistry in lung tissue, TIMP-1 MMP-9.Result: The clinical research results show that, the clinical symptoms and pulmonary function in the treatment group with combination of TCM and Western medicine was significantly better than western medicine treatment group and significantly improved than before treatment. Animal experimental results show that the centipede and pirfenidone groups have strong effect in the therapy of interstitial lung disease.The effect is obviously better than that of Jodison +N-acetylcystein e+azathioprine group. The experiment also found that, different concentrations of centipede have different roles in different indicators of intervention. In the transforming growth factor beta 1, vascular endothelial growth factor, matrix metalloproteinase-9 and tissue inhibitor-1, interleukin-8, the concentration of low concentration of centipede, centipede, pirfenidone group, compound group than in high concentration treatment of centipede has a more active role. In the index of oxidant / antioxidant, little difference in the effect of combination of centipede pirfenidone, were better than those of composite group. The intervention effect of type I collagen, high concentration centipede and pirfenidone intervention than other groups.Conclusion:Through the clinical observation, we found in Huoxuetongluo formula as the representative of the traditional Chinese medicine in the treatment of phlegm stasis Bizu interstitial lung disease can significantly improve the symptoms. Traditional Chinese medicine has obvious advantages in improving the quality of life of patients. Through the search by the animal experimental study on promoting blood circulation drugs centipede, centipede in inhibit transforming growth factor and vascular endothelial growth factor mRNA expression and inhibition of oxidation reaction and reduced collagen deposition, and reduce the inflammatory reaction, the inhibition of matrix metalloproteinase and prevent remodeling of the extracellular matrix has a significant intervention effect. This study provides a theoretical basis for clinical treatment, and provides ideas for drug development. |
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