Research About Yangjin Hufei Decoction Affect On Human Lung Cancer Cell A549 Mechanism Of Proliferation,apoptosis

Objective: by Yangjin hufei soup on experimental study of induced apoptosis and inhibit the proliferation of lung cancer cell line A549, to learn the effect and mechanism of the treatment of advanced lung cancer, and provide a modern, scientificbasis in the clinical application.Methods: by supplementing qi and nourishing Yin Runfei, Huatan Quyu Jiedu for therapy of traditional Chinese medicine, modern experimental research for mechanism of raising gold protecting lung decoction in the treatment of lung cancer.(1) By means of four methyl thiazolyl tetrazolium(MTT) colori metric method, which contrast 10mg/ml, 5mg/ml, 2.5mg/ml, 1.25mg/ ml, 0.625mg/ml nutrient gold protecting lung soup in 24 h, 48 h, 72 h act on human lung cancer cell line A549 growth inhibition effect as chemotherapy drug cisplatin 150ug/ml acts on people lung cancer cel l growth inhibition of A549 phase;(2)Through AO/EB dyeing watch cell morphology and ultrastructure of human lung cancer cell line A549 change;(3)Using Real-ti me Quantitative PCR Detecting System(RT-q PCR) to observe raise gold protecting lung soup affect related apoptosis gene Bcl-2 and tumor suppressor gene the regulation of P53 expression, which explore its anti tumor effect in molecular mechanism.Results: By means of four methyl thiazolyl tetrazolium(MTT) colorimetric method, which contrast 10mg/ ml, 5mg/ ml, 2.5mg/ml, 1.25mg/ml, 0.625mg/ml nutrient gold protecting lung soup in 24 h, 48 h, 72 h act on human lung cancer cell line A549 growth inhibition effect as che motherapy drug cisplatin 150ug/ml acts on people lung cancer cell growth inhibition of A549 phase, whic suggest that raising gold protecting lung decoction on A549 lung cancer growth inhibition with increasing drug concentration and action time increased, showed time and dose dependent effect;(2)morphological observation: AO/EB staining showed that the protective effect on lung soup Yang Jin lung A549 cells, the Chinese medicine group of early apoptotic cells slightly more than that in the control group, as the dose increased, late apoptotic and dead cells gradually increased;(3) RT-q PCR: compared with the control group, Bcl-2 gene and P53 of diffe rent concentrations of drugs in the experimental group A549 cells in the expression levels were rising trend, and show a certain dose effect relationship. The experimental results by analysis, confirmed that raise gold protecting lung decoction has the inhibition of human lung cancer A549 cells proliferation and induce its apoptosis inhibition, preli minarily suggested raising gold protecting lung decoction on lung cancer cell s through molecular biological pathways regulating cell proliferation, apoptosis, which may influence the development and transforma tion of lung cancer.