Study On A Bistratal Artificial Blood Vessel And Its Biocompatibility

In clinical application, although the demand for small diameter vascular (less than or equal to 6mm) larger, but in small diameter (<6mm) vascular grafts, mid- and long-term outcome of many synthetic tissue engineering stent is not good, often because of the lack of endothelial function in low flow, forming thrombus, aneurysm, intimal hyperplasia, but is defeated comes to an end. Therefore, looking for a manufacture method can maintain its normal physiological function in vivo tissue engineering stent for a long time, the small diameter vascular tissue engineering is an important problem to be solved, but the natural acellular vascular, the advantage is that, apart from the extracellular matrix cell has the following characteristics:1. Provide the biomechanical properties of scaffold, and to maintain a certain strength and specific shape; as an alternative cell proliferation and migration of bioactive scaffolds; the possible regulation of cell phenotype and cell behavior; and as an anchor point, as a growth factor and a variety of enzyme or protein, provide reaction sites. Specific.This topic in the laboratory prepared RGD-(Recombinant spider silk protein spider silk protein, pNSR32), poly caprolactone (polycaprolactone, PCL), gelatin (Gelatin, Gt) basis of preparation of pNSR32/PCL/Gt small diameter vascular stent, the biocompatibility of natural extracellular matrix, acellular vascular construction-(PCL/Gt/pNSR32) double stent to treatment, detergent, detergent and protease treatment method, enzyme treatment method and freeze-thaw method, were investigated on the removal of cells, through frozen sections, DAPI staining followed by hematoxylin eosin staining, DNA method, residue rate determination the removal efficiency of cell detection. Combined with the room of prepared RGD spider silk protein electrostatic/PCL/Gt spinning frame foundation, using electrostatic spinning technology of preparation pNSR32/PCL/Gt (5:85:10) electrospun membrane. At the same time, by containing 0.25% glutaraldehyde lOmmol/L acetic acid solution and preparation of removal of vascular cell scaffold crosslinking, respectively crosslinking at 6h,12h,24h respectively (named a, group B and group C), constitute a distinct structure, the obvious functional double vascular scaffolds. The porosity results showed that the porosity of the scaffolds was in accord with the national standards, and was more than 80%, and the crosslinking time was negatively correlated with the crosslinking time.. At the same time, the contact angle of the scaffold increased with the increase of the crosslinking time, indicating that the hydrophilicity of the scaffold was gradually decreased.. In addition, the suture strength of double layer vessel scaffold increased with the increase of crosslinking time, but the elongation of the scaffold decreased with the increase of the crosslinking time.. At the end of the investigation found that the blood compatibility of hemolysis in hemolysis test in A group, B group, C group support rates were 1.299%,1.818%,3.247%, accord with the requirement of less than 5% of the hemolytic rate of hemolysis in the national standard; hemolysis double stent in each experimental group were lower than 4%, A group and B group respectively. C group was 1.299%,1.818%,3.247%, to meet the vascular tissue engineering scaffold material requirements for hemolysis; recalcification clotting time A, B two group is more outstanding, and there was no significant difference among A group, B group and group support bracket for anticoagulant properties of intrinsic coagulation pathway is similar to that of the stent was better than that of group C; dynamic clotting time test and platelet adhesion test results and two experimental results. In summary, A group support is in the three experimental groups anti clotting properties of the most excellent support. Removing cell vascular stent (PCL/Gt/RGD-spider silk protein) double stent blood compatibility decreased with the extension of glutaraldehyde reaction time. In a word, de vascular cell scaffold/(PCL/Gt/RGD-spider silk protein) double stent anticoagulant ability is excellent, with good vascular tissue engineering scaffold application prospect.