Study On The Detection Of Several Antibiotics Based On Surface Enhanced Raman Scattering

Objective To study Benzylpenicillin sodium(NaBP) and Ciprofloxacin(CIP) by using Raman and Surface-enhanced Raman scattering(SERS). And focuses on studying the interaction mechanism between the two drug molecules(NaBP and CIP) and the two substrates(Ag TiO2) respectively. And research the influence of the interaction between drug molecules and substrates by changing the other factors such as drug concentration, particle size, reaction temperature, pH value, adsorption time and so on.Methods To compare the normal Raman of the solid drug molecules and the Surfaceenhanced spectroscopy Raman(SERS) of the drug molecules adsorbed on the substrates. The characteristic peaks were analyzed and characterized. And analyze the SERS signals which are obtained under the different parameters such as adsorption time, drug concentration, pH value and so on.Results NaBP is close to the silver surface through the carboxyl bond, the detection limit can be reduced to 10-7 mol/L by SERS method. Importantly, there is a good linear relationship in the range of 10-4~10-7 mol/L concentration(R2=0.96256), and a quantitative detection method of benzylpenicillin sodium can be established. In addition, it is proved that NaBP is not stable under the conditions of strong acid and alkali, which can be identified whether there are degradation products by using SERS technology and reduce the occurrence of allergic source. Ciprofloxacin is connected to the TiO2 surface through the carboxyl group, and making the Raman signal enhancement of the CIP molecule and TiO2 substrate through the charge transfer(CT) and the phonon mode vibration of TiO2 respectively. We can obtain the maximum SERS spectra of CIP adsorbed on TiO2 NPs when the calcination temperature is 450?C, the adsorption time is 9 h and the pH value of solution is 5~7.Conclusions Raman and Surface-enhanced spectroscopy Raman are successfully used for detection and analysis of antibiotics, provide a new analysis method for the detection of Benzylpenicillin sodium(NaBP) and Ciprofloxacin(CIP). We also provide the references and laid a solid foundation for the analysis of the other antibiotics in the future.