Construction And Application Of Surface-Enhanced Raman Scattering Tags For Tumor Cell Diagnosis And Treatment

Malignant tumor is a serious threat to human health and life. The development of efficient tumor diagnosis and treatment technology to reduce the fatality rate of malignant tumor has great significance to improve the life quality of patients. With the development of nanotechnology, nanomaterials showgreat potential in the diagnosis and treatment of tumors. Recently, design and preparation of diagnostic nanomaterials have become a research hotspot, however, it still exists some problems and technical difficulties in this field. In this thesis, we foucs on the development of multi-functional targeted nanoprobes for SERS imaging and photothermal therapy(PTT) for tumor, aiming to provide several new nanoplatforms for detection and treatment of malignant tumors. Gold nanoparticles and multifunctional probes have been synthesized and been applied in SERS imaging and PTT. The five chapters are summarized as follows:In the first chapter, the research background is summarized. Briefly, the current clinical diagnosis and treatment of tumor were introduced, and the advantages of SERS imaging and PTT treatment based on plasmonic nanomaterials were put forward.In the second chapter, a novel kind of flower-like gold nanoparticles synthesized by a simple method is proposed. The proposed gold nanoflowers with the surface occupied by many nanogaps, known as SERS "hot spots", show excellent SERS activity. The gold nanoflowers were further labeled with Raman molecules and functionalized by biological molecules to be SERS tags for targeted imaging of lung cancer cells.In the third chapter, a new kind of star-shaped gold nanoparticles were controlled synthesized by a seed-mediated growth method. The gold nanostars with several branches can strongly absorb near-infrared light. By the same biological functionalization mentioned above, the SERS tags can be used for targeting and SERS imaging of A549 lung cancer cell.In the fourth chapter, the photothermal properties of Au nanomaterials with different morphologies were studied and the optimal heating result was obtained from the star-shaped gold naoparticles when they were irradiated by 785 nm laser. Furthermore, the therapeutic effect of the SERS tags in vitro was investigated, which indicates that the proposed SERS tags have good performance in diagnosis and treatment of tumor.In the last chapter, the conclusions and prospect of the present work were summarized.