The Study On Intervention Of Lycopene To Rats Lung Injury Caused By Rare Earth Nd₂O₃ Particles

Chapter 1 Research on rats lung injury caused by rare earth neodymium oxide particlesObjective To explore the relationship between the change of inflammatory cells in the bronchoalveolar lavage fluid(BALF) and the lung histopathology findings, the content distribution of neodymium oxide in rat liver, brain and kidney. Method Seventy two SD rats were randomly divided into four experimental groups: the control group and the three neodymium oxide treated groups(25, 50, 100 mg/kg). Disposable non-exposed type intratracheal perfusion was adopted to make the rat lung injury model. At the 14, 21 and 28 days, BALF was collected by bronchoalveolar lavage. Total cell count and differential cell counts in BALF were estimated and paraffin-embedded lung sections with HE staining was observed under light microscope. ICP-MS was used to measure the neodymium oxide content in the rat liver, brain and kidney. Results At the 14、21、28 days, lung histopathology showed that the alveolar was in disordered arrangement, the alveolar interval widened by those of inflammatory cells infiltrated in the early stage of the lung injury, which was followed by the cell nodule formation and diffuse into the film. The number of total cells and the absolute number of differential cells, including lymphocytes, neutrophils, macrophages in the BALF were elevated in Nd2O3-treated groups(P<0.05). Neutrophils and macrophages were elevated in the early phase, lymphocytes was elevated in late phase. Lung histopathology showed the same findings as in BALF. Compared with the control group, neodymium oxide content of the rat liver, brain and kidney was higher, the result show neodymium oxide content of rat liver was higher than those of the brain and kidney(P<0.05). Conclusions Rare earth neodymium oxide particles caused rats lung injury. Mainly inflammatory injury in the early, cells nodules forming in the late. Neodymium oxide particles redistributed between the rat liver, kidney and brain through respiratory tract infected. The neodymium oxide content in liver is higher than those of the kidney and brain.Chapter 2 The Study on Intervention of Lycopene to Rats Lung Injury Caused by Rare Earth Nd2O3 ParticlesObjective To observe the intervention of lycopene to rats lung injury caused by rare earth neodymium oxide particles. Method Ninety SD rats were randomly divided into five experimental groups: the control group, the positive control group and the three lycopene treated groups(1, 3, 5 mg/kg). Disposable non-exposed type intratracheal perfusion was adopted to make the rat lung injury model. After exposed, the lycopene low, medium and high dose group intervented with lycopene solution diluted with soybean salad oil. With equal volume of ranging lycopene concentrations, 0.5 ml/100 g body mass solution to rats once a day by intragastric administration, while giving the control group and the positive control group with equal volume of soybean salad oil. At the 28 and 56 days, serum was separated and collected by the blood from the abdominal aorta. Paraffin-embedded lung sections with HE staining was observed under light microscope. Rat lung homogenates collected to measure the content of oxidation-antioxidant indicators including SOD, MDA, GSH-Px, HYP and the CC16 and SP-D in serum. Results At the 28 and 56 days, compared with the control group, the weight in each group were reduced, the lycopene-treated group was higher than the positive group(P<0.05). The rats lung coefficient in each group was increased, and the positive group was higher than the lycopene-treated group(P<0.05).The content of SOD and GSH-Px in the lycopene-treated group was higher than the positive group(P<0.01).The MDA and HYP content of the positive group was higher than the control group and the lycopene-treated group(P<0.01).Compared with the positive group, the content of CC16 in serum of the control group was lower(P<0.01), the lycopene-treated group was significantly increased(P<0.01), and the SP-D content in serum of the control group was lower(P<0.01), the lycopene-treated group was significantly reduced(P<0.01). Conclusions Lycopene can significantly improve the vitality of antioxidant enzymes and the CC16 content, reduce the lung collagen content and SP-D. Lycopene can enhance the ability of body’s antioxidant and scavenge free radicals, reduce the degree of lung injury and hinder the development of pulmonary fibrosis. Lycopene has a positive protectiveeffection in rat lung injury caused by the Nd2O3 particles.