The Expression Of Notch-1 Protein In Severe Preeclampsia Placentas And The Relationship With Maternal And Perinatal Outcomes

Objective: To evaluate the expression of Notch-1 protein in normal and preeclampsia placentas, and to analyze the relationship with maternal and perinatal outcomes for providing biological evidence for early clinical intervention of preeclampsia.Methods: One hundred and nine cases meeting the criteria of patients placentas were enrolled in this study, these cases were collected from the First Affiliated Hospital of Henan University of Science and Technology in November 2012 to February 2014. It contains 30 patients with early oneset severe preeclampsia(ESPE) < 34 weeks and 30 cases with late onset severe preeclampsia(LSPE) 34 weeks, no complications of pregnant placenta tissue in 49 cases, 23 cases with no complications of preterm pregnant women and normal full-term pregnant women 26 cases. Immunohistochemical method were used to detect the expression level of Notch-1 protein. Collect and follow-up the pregnancy complications and perinatal outcomes of 60 cases with severe preeclampsia. SPSS17.0 software was used for data analysis.Results: 1. The expression of Notch-1 protein in placenta: Notch-1 protein mainly located in the placenta of sertoli cell membrane, and also have a small amount of expression in the cytoplasm as well as placental blood vessel cells.2. The strong expression of Notch-1 protein in normal placenta was 75.5%, weak expression is 24.5%; In severe preeclampsia strong expression was 46.7% and weak expression was 53.3%. Use the chi-squared test to analyze the difference of Notch-1 protein expression, there was significant difference in two groups(P<0.05).3. Compared with early onset severe preeclampsia placenta expression, Notch-1 protein expression in the preterm birth without complications was significant stronger than early onset severe preeclampsia(P<0.05). The expression of Notch-1 protein in normal term pregnancy was stronger than late onset severe preeclampsia(P<0.05). Meanwhile, the expression of Notch-1 protein in early onset was weaker than late onset severe preeclampsia(P<0.05).4. Systolic blood pressure of early-onset group on admission was significantly higher than late onset group, and the result was statistically significant(P=0.034). Diastolic blood pressure of early-onset group on admission was also significantly higher than late onset group, and the result was statistically significant(P=0.018).5. There are 35 cases with one or more severe maternal clinical complications in the 60 cases of severe preeclampsia, among them, 22 cases belong to the early-onset group, 13 cases of late onset group, complication incidence in early-onset group(73.3%) was strongerer than the late onset group(43.3%), and the result was statistically significant(P<0.05). There are 26 cases with one or more births infant complications in the two groups, 17 cases belong to early onset group, 9 cases in late onset group, compared complication incidence between the two groups, the result was statistically significant(P<0.05).6. To analyze the relationship between different expression of Notch-1 protein in severe preeclampsia and the maternal clinical complications, the result shows that(P< 0.05); compared the expression of Notch-1 protein in late onset severe preeclampsia with maternal clinical complications, the result shows that the more amount expression of Notch-1 protein in placental tissue, the lower of maternal clinical complications incidence(P<0.05). To analyze the relationship between different expression of Notch-1 protein in severe preeclampsia and perinatal outcomes, the result shows that the more amount expression of Notch-1 protein in placental tissue, the lower of perinatal outcomes(P<0.05).Conclusions: 1. The less amount expression of Notch-1 protein in placental tissue, the heavier of maternal clinical complications; the earlier the onset of preeclampsia, the less amount expression of Notch-1 protein in placental tissue.2. Comparison of the two groups of maternal and perinatal outcomes, suggesting that the earlier the onset of preeclampsia, the worse of maternal and perinatal outcomes.3. To analyze the relationship between different expression of Notch-1 protein in severe preeclampsia and the maternal perinatal outcomes, the result shows that the expression of Notch-1 protein in the placenta has a relationship with maternal and perinatal outcomes, the lower Notch-1 protein expression, the higher maternal and perinatal complications, and the maternal and perinatal outcomes is worse.