| Objective: The use of non-alcoholic steatohepatitis rats were observed under the effect of Ginkgo biloba extract objects rats TLR4,TNF-α expression. Methods: The 45 male SD rats were randomly divided into three groups, the control group, model group and treatment group, n = 15. Control rats given a normal diet. Model group and the treatment group were given high-fat diet, after 12 weeks of feeding induced nonalcoholic steatohepatitis,Model group with saline l0 mL / kg orally, Treated with ginkgo biloba extract liquid l0 mL / kg orally,Each of two groups of rats fed once a day, a total of eight weeks. After the rats were anesthetized and its blood and liver tissue samples taken, the rats were killed. All samples were below detection:(1) the use of automatic biochemical analyzer to detect the serum alanine aminotransferase, aspartate aminotransferase levels, using radioimmunoassay of serum TNF-α levels.(2) ELISA method to measure the level of TLR4 protein expression, RT-PCR was used to detect the expression level of TLR4.(3) HE staining of liver tissue samples of liver pathological changes under light microscope; Results:(1) Weight of the treatment group and the model group was significantly increased,Weight faster growth rate than the control group. Model group and the treatment group had varying degrees of hepatic steatosis in liver tissue changes in the optical microscope to see, and treatment of hepatic steatosis cases lighter than the model group, Control group without fatty liver.(2) Model group and treatment group TLR4 mRNA and protein expression levels and serum TNF-α expression were higher than The control group,and The expression level of the model group was higher than the treatment group.(3)The serum ALT, AST of Model group and treatment group levels were significantly higher than the control group,and The treatment group ALT, AST levels were lower than the model group, the difference was statistically significant. Conclusion: Pathology treated rats steatohepatitis degree lower than the model group, And the treatment of liver function parameters, TNF-α,TLR4 mRNA and the expression of protein were lower than the model group. Ginkgo biloba extract on rats with nonalcoholic fatty liver hepatitis protective effect to some extent, It’s possible mechanism is to reduce the expression of TLR4, TNF-α, Inhibit the inflammatory response. |
