Protective Effect Of Betaine On Acute Myocardial Ischemia Via Inhibition Of Inflammation Factor In Rats

Objectives:(1) To study the protective effect of betaine on isoproterenol- induced myocardial ischemia injury and the regulation on the the expression of inflammatory factors in rats with 40 days administration.(2) To investigate the protective effect and mechanism of betaine on isoproterenol-induced myocardial ischemia injury in rats with 10 days administrationMethods:(1) Sprague-Dawley rats were divided into six groups: Control group, model group(isoproterenol(ISO) 85 mg.kg-1), betaine100 mg.kg-1+ISO group, 200 mg.kg-1+ISO group, 400 mg.kg-1+ISO group, betaine 400 mg.kg-1 group. The rats in treated and betaine groups were given betaine by oral administration once a day for 40 days, then the rats in model and treated groups were given subcutaneous injection of ISO 85 mg.kg-1.d-1 separately after they were given bataine on the 39 th and 40 th day, and in this way the cute myocardial ischemic model were established. Then the left ventricular apical histomorphological changes were observed by light microscope, and the expression of inflammation factors in left ventricle were measured by protein-chip technique and enzyme-linked immunosorbent assay.(2) Rats groups were same with(1). The rats in treated and betaine groups were given betaine by oral administration once a day for 10 days, then the rats in model and treated groups were given subcutaneous injection of ISO 85 mg.kg-1.d-1 separately on the 9th and 10 th day, and the cute myocardial ischemic model were established. Then the left ventricular apical histomorphological changes were observed, and the injured cardiac muscular tissue marker enzymes such as lactate dehydrogenase(LDH), glutamic-oxalacetic transaminase(GOT), creatine kinase(CK) were measured, and glutathione peroxidase(GSH-PX), catalase(CAT) and malondialdehyde(MAD) were also measured.Results:(1) Give rats betaine for 40 days: Compared with the model group, the betaine treated groups can improve the cardiac histomorphological changes at different levels, and oral administration of betaine(200, 400 mg·kg-1·d-1) can significantly decrease the high expression of MCP-1 and ICAM-1; compared with the control group, the expression of inflammation factors such as ICAM-1, MCP-1,β-NGF, IL-1β, IL-4, TIMP-1 and VEGF is different in the model group.(2) Give rats betaine for 10 days: Compared with the model group, the betaine treated groups can improve the cardiac histomorphological changes, and oral administration of betaine(400 mg·kg-1·d-1) can significantly decrease the content of LDH, GOT and CK in serum, and oral administration of betaine(200 mg·kg-1·d-1) can significantly decrease the content of LDH and CK; each measurement in betaine 400 mg.kg-1 group is not significantly different with that in the control group; oral administration of betaine(200 or 400 mg.kg-1.d-1) can significantly increase the content of GSH-PX and CAT and reduce the content of MDA in the serum; oral administration of betaine(400 mg·kg-1·d-1) can significantly increase the content of GSH-PX and CAT and reduce the content of MDA in the rat myocardium, and oral administration of betaine(200 mg·kg-1·d-1) can reduce the content of MDA in the rat myocardium.Conclusions:(1) Give rats betaine for 40 days, the protective effect of betaine on isoproterenol-induced acute myocardial ischemia is related to inhibition of MCP-1 and ICAM-1.(2) Give rats betaine for 10 days, the protective effect of betaine on isoproterenolinduced acute myocardial ischemia is related to antioxidation.