| Since Traditional Chinese medicine has the advantages of toxic and side effects of small and fewer adverse reactions on tumors treatment, TCM has obvious advantages compared with western medicine lily is a very common plant, in addition to the ornamental value, it has a good medicinal value, lily was first approvaled that it has a good medicinal value and has a good edible value as a plant by Ministry of Health, its many active ingredient malathion was confirmed by some study that it has excellent effects at many aspects of anti-tumor, strengthening organism immune system, treatment of symptoms after radiation, etc, such as colchicine—one ingredient of lily was confirmed that it has anti tumor effects. Now it has been reported that many active ingredient malathion of lily has anti tumor effects by some literatures, but its functional mechanism is not clear and bright. Gastric cancer is one of malignant tumors which can harm human health, because its early detection rate is low, so chemotherapy has a vital role in the treatment process of patients with most of the gastric cancer. Now the study was not be reported that the effects of lily on Human Gastric Cancer Cell Lines SGC-7901, so in this study, the proliferation inhibition of lily extract alkaloid and carbinol extract on Human Gastric Cancer Cell Lines SGC-7901 and discussion preliminary of functional mechanism is studied.Objective:This experiment analyzed that effects of lily extract alkaloid and carbinol extract on proliferation inhibition and apoptosis of SGC-7901 Cell, discussion of the possible mechanism of action.Methods:This study used MTT method to detect the inhibition ratio of different concentration of lily extract alkaloid and carbinol extract on SGC-7901 Cell for 24h,48h and 72h; Inverted phase contrast and fluorescence microscope observed cellular morphology change after AO/EB staining; Flow cytometry detected the change of cell cycles of SGC-7901 Cell and apoptosis rate of SGC-7901 Cell; Western blotting detected the expression of caspase-3 protein of SGC-7901 Cell.Results:1. The result of MTT showed that different concentration of lily extract alkaloid and carbinol extract can inhibit the growth and increasing of SGC-7901 Cell observably, the inhibition ratio is 21.5%,28.27%,37.13% respectively after 24h,48h, 72h following lily extract alkaloid of 0.05 g/L on SGC-7901 Cell, the inhibition ratio is 31.87%,40.63%,49.30% of 0.075g/L respectively, the inhibition ratio is 40.90%, 50.13%,58.10% of 0.1 g/L respectively, the inhibition ratio is 51.33%,65.60%,80.07% of 0.125g/L respectively, the inhibition ratio is 65.30%,74.06%,91.93% of 0.15g/L respectively, the inhibition ratio is 5.53%,14.58%,23.39% respectively after 24h,48h, 72h following carbinol extract of 0.8 g/L on SGC-7901 Cell, the inhibition ratio is 19.62%,30.34%,37.23% of 0.9g/L respectively, the inhibition ratio is 27.26%,38.73%, 46.76% of 1.0g/L respectively, the inhibition ratio is 35.85%,45.84%,53.31% of 1.1g/L respectively, the inhibition ratio is 42.23%,52.32%,58.39% of 1.2g/L respectively, the inhibition ratio is 50.29%,58.31%,66.47% of 1.3g/L respectively, the inhibition ratio is 59.36%,69.32%,82.69% of 1.4g/L respectively, the inhibition ratio is 69.39%,86.15%, 96.74% of 1.5g/L respectively, the inhibition ratio is 76.33%,96.74%,98.32% of 1.6g/L respectively, the result shows time and dose dependent.2. Following different concentration of lily extract alkaloid and carbinol extract treatment of SGC-7901 Cell for 48h, after AO/EB staining of SGC-7901 Cell, fluorescence microscopy observed that down-slowed cell growth, emergence of apoptosis, normal cells and early apoptotic cells, late apoptotic cells and necrotic cells.3. Flow cytometry analysis of cell cycle showed that G2/M period block of SGC-7901 Cell, the content of G2/M period is 10.70%,15.81%,20.90%,30.02% respectively after 48h following blank control group,0.05 g/L of lily extract alkaloid,0.1 g/L of lily extract alkaloid and 0.15 g/L of lily extract alkaloid on SGC-7901 Cell, the content of G2/M period is 10.70%,13.31%,19.99%,27.03% respectively after 48h following blank control group,0.9 g/L of carbinol extract,1.1 g/L of carbinol extract and 1.4 g/L of carbinol extract on SGC-7901 Cell, which were dose dependent.4. Flow cytometry analysis of cell apoptosis rate showed that apoptosis rate of SGC-7901 Cell is 1.57%,6.54%,11.14%,20.35% respectively after 48h following blank control group,0.05 g/L of lily extract alkaloid,0.1 g/L of lily extract alkaloid and 0.15 g/L of lily extract alkaloid on SGC-7901 Cell, apoptosis rate of SGC-7901 Cell is 1.57%, 5.36%,11.78%,20.32% respectively after 48h following blank control group,0.9 g/L of carbinol extract,1.1 g/L of carbinol extract and 1.4 g/L of carbinol extract on SGC-7901 Cell, which were dose dependent.5. Western blotting observed that caspase-3 protein expression in SGC-7901 Cell and the expression quantity increased gradually with the increase of drug concentration gradually.Conclusion:1. Different concentration of lily extract alkaloid and carbinol extract can significantly inhibit SGC-7901 Cell from proliferation with time and dose dependent.2. Can change the cycle and make SGC-7901 Cell block in the G2/M period.3. Can induce apoptosis of SGC-7901 Cell. Can raise caspase-3 protein expression in SGC-7901 Cell, it can be one of important functional mechanism of inducing SGC-7901 Cell apoptosis. |
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