The Changes Of Th17 Cells And Treg Cells And Tfh Cells In Peripheral Blood With Hashimoto’s Thyroiditis

Objective: To observe follicular helper T cells(Tfh)and helper T cell(Th)17 and CD4+CD25+regulatory T cell(Treg) in peripheral blood of patients with Hashimoto’s thyroiditis(HT) and Graves disease(GD),to explore the immune mechanism of HT.Methods:50 HT patients and 36 GD patients selected from outpatient department and inpatient department in March 2013 to December 2014 and healthy subjects with age and gender matched in our hospital.The Tfh cells and Th17 cells and Treg cells in the peripheral blood of three group examed by flow cytometry and the ratio of Tfh/CD4+T and Th17 / CD4+ T and Treg/CD4 + T cell calculated.Result:(1) The percentage of Tfh/CD4 + T cell in HT patients was(14.31+2.19) %. The percentage of Tfh/CD4 + T cells in GD patients were(12.98+3.61) % they were both higher than that in healthy subjects((6.35+1.11) %), but there were no statistically significant difference between HT patients with GD patients(P > 0.05).(2) The percentage of Th17/CD4 + T cell in HT patients was(2.01+0.48) %. The percentage of Th17/CD4 + T cells in GD patients was(1.90 + 1.00) %, and they were both higher than that in healthy subjects with physical examination((0.57+0.33) %), There were statistically significant difference(P < 0.05), but there were no statistically significant difference between HT patients with GD patients(P > 0.05).(3) The percentage of Treg/CD4 + T cell in HT patients were(2.90+1.04) %. The percentage of Treg/CD4 + T cells in GD patients were(2.58 + 1.51) %, and the two percentages were both lower than that in healthy subjects with physical examination((4.43+0.57) %), There were statistically significant difference(P < 0.05), but there were no statistically significant difference between HT patients with GD patients(P > 0.05).(4) In HT patients, there was significant correlation between the positive expression of thyroid autoantibody(TPOAb、TGAb) and the changes of Tfh cells subpopulations(r=0.31,p<0.05;r=0.33,p<0.05), significant correlation between the positive expression of thyroid autoantibody(TPOAb)and the changes of Th17 cells subpopulations(r=0.43,p<0.05), negative correlation between the positive expression of thyroid autoantibody(TPOAb) and the changes of Treg cells subpopulations(r=0.3,p<0.05).Conclusion:The percentage of Tfh/CD4 + T cell and Th17/CD4 + T cell in HT patients and GD patients were both higher than that in healthy subjects. There was significant correlation between the positive expression of thyroid autoantibody(TPOAb,TGAb) and the changes of Tfh cells subpopulations in HT patients, significant correlation between the positive expression of thyroid autoantibody(TPOAb) and the changes of Th17 cells subpopulations, The percentage of Treg/CD4 Tcell in HT and GD were both lower than in healthy subjects with physical examination. In HT patients showed negative correlation between the positive expression of thyroid autoantibody(TPOAb) and the changes of Treg cells subpopulations.The three most important helper cells for clinical diagnosis of HT related antibody TPOAb, TGAb related, it also suggests that by detected Tfh cells and Th17 cells and Treg cells help to understand the pathogenesis of HT.