| Objective:To compare the allele distributions and genotype frequencies about SNPs(rs2292832, rs11614913, rs3746444) in the region of mi R-499, mi R-196a2 and mi R-149 between SLE patients and normal controls, RA patients and normal controls; and investigate the association between mi R-499, mi R-196a2 and mi R-149 polymorphisms with SLE, RA susceptibility; and analyze the relationship between mi R-499, mi R-196a2 and mi R-149 polymorphisms with clinical characteristics, autoantibodies in SLE, RA patients. Methods:120 SLE patients and 120 RA patients were recruited from Department of Rheumatology and Immunology of the Affiliated Nanjing Hospital of Nanjing Medical University. All SLE and RA patients were according to the 1997 revised American College of Rheumatology(ACR) classification criteria and diagnosed by the two director level and above specialists.120 normal controls were recruited from healthy volunteers, all of them fulfilled these criteria: without one of the diagnostic criteria for SLE and RA; themselves and their immediate family members without a history of autoimmune disease; healthy in the last month and no use of hormones and immunosuppressant drugs, no history of major diseases. After obtaining the informed consent, we collected clinical data and 2 ml anti-coagulated venous blood samples from all studied subjects, and extract whole blood genomic DNA. SNPs of mi R-499 rs2292832, mi R-196a2 rs11614913, and mi R-149 rs3746444 were detected by PCR-RFLP(polymerase chain reaction-restriction fragment length polymorphism). SPSS 17.0 software was used for the data analysis. The chi-square test was used for the data analyze of the measurement data. Logistic regression analysis was used to calculate odd ratios(ORs) and P values with adjustment for gender, age, etc. Hardy-Weinberg equilibrium was calculated by SPSS 17.0. The statistical significance defined as P value<0.05 and calculated based on two-sided tests.Results:(1)Association analysis of mi RNA gene SNPs and SLE susceptibilityTo SNP rs11614913 of mi R-196a2, genotype frequencies for TT, TC and CC were 10.8%, 22.5% and 66.7% in patients; and 15.8%, 27.5%, 56.7% in the normal controls. The onset risk of allele C in SLE patients was increased compared to allele T.OR= OR=1.28, 95%CI(1.03-1.84), P=0.017.The mi R-196a2 polymorphism was related to the onset risk of SLE patients.To SNP rs22928323 of mi R-149, genotype frequencies for TT, TC and CC were 25.2%, 28% and 46.8% in patients; and 27.5%, 21.7%, 50.8% in the normal controls. The onset risk of allele C in SLE patients was not increased compared to allele T. OR=1.38,95%CI(1.01-1.75), P=0.023。The mi R-149 polymorphism was not related to the onset risk of SLE patients.To SNP rs3746444 of mi R-499, genotype frequencies for AA, AG and GG were 19.8%, 29.7% and 50.5% in patients; and 18.3%, 29.2%, 52.5% in the normal controls. The onset risk of allele G in SLE patients was not increased compared to allele A. OR=0.78, 95%CI(0.58-1.11), P=0.188. The mi R-499 polymorphism was not related to the onset risk of SLE patients.(2)Association analysis of mi RNA gene SNPs and RA susceptibilityTo SNP rs11614913 of mi R-196a2, genotype frequencies for TT, TC and CC were 26.9%, 21.5% and 51.6%; and 21.6%, 26.7%, 51.7% in the normal controls. The onset risk of allele C in RA patients was not increased compared to allele T. OR=0.69,95%CI(0.24-1.18), P=0.248 the mi R-196a2 polymorphism was not related to the onset risk of RA patients.To SNP rs22928323 of mi R-149, genotype frequencies for TT, TC and CC were 25.3%, 51.1% and 23.6%; and 22.5%, 26.7%, 50.8% in the normal controls. The onset risk of allele C in SLE patients was increased compared to allele T. OR=1.38,95%CI(1.01-1.75), P=0.023。The mi R-149 polymorphism was related to the onset risk of SLE patients.To SNP rs3746444 of mi R-499, genotype frequencies for AA, AG and GG were 12.9%, 19.9% and 67.2%; and 18.3%, 20.0%, 61.7% in the normal controls. The onset risk of allele G in SLE patients was not increased compared to allele A. OR=0.75, 95%CI(0.54-1.17), P=0.159. The mi R-499 polymorphism was not related to the onset risk of SLE patients.(3)Association of mi RNA SNPs and SLE clinical manifestationThe anti-ANA, anti-ds DNA, anti-Sm, anti-ACA, PLT, C3, C4 in SLE patients were not related to the three mi RNA SNPs.(P>0.05)4) Association of mi RNA SNPs and RA clinical manifestationThe AKA, CCP, RF, WBC, BUN, Cr in RA patients were not related to the three mi RNA SNPs.(P>0.05) Conclusion:(1)SNP rs11614913 in mi R-196a2 is correlated with susceptibility to SLE in East Chinese Han population.(2)SNP rs22928323 in mi R-149 is correlated with susceptibility to RA in East Chinese Han population... |
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