| Objective:The aim of this study was to observe if IETM was existed or not in AD patients and to investigate the effect of IETM in AD, provide evidence for the prevention and treatment of AD.Methods:According to the inclusion and the exclusion criteria, choose AD patients and healthy eldly, evaluate cognition by the MMSE and ADAS-cog, detect the serum LPS, TNF-α and Aβ level by ELISA, detect APP, PS1 and BACE m RNA expression by real-time PCR. All the data were analyzed by SPSS 17.0.Results:1.The AD group and the control group showed no significant differences in sex(χ2=0.312, P=0.576), age(t=0.243, P=0.809) and education level(u=735.000, P=0.682).2.The MMSE score of AD group was significantly lower than the control group(u=0.000, P<0.001), the ADAS-cog score was significantly higher than that in control group(u=0.000, P<0.001), the differences were statistically significant.3.The LPS(u=0.000, P<0.001), TNF-α(t=6.175, P<0.001), Aβ(u=13.000, P<0.001)levels were significantly higher than the control group, the differences were statistically significant.4.The APP(u=16.000, P<0.001), PS1(u=24.000, P<0.001) and BACE(u=60.000,P<0.001) m RNA expression levels in AD group were significantly higher than the control group, the differences were statistically significant.5.The LPS level was highly related to the Aβ level(r=0.894), The LPS level was moderately related to the APP(r=0.563), BACE(r=0.486) m RNA expression. The correlation between LPS level and PS1 m RNA expression was not significant.Conclusions:This study preliminary confirmed that AD patients occurs IETM, and IETM could upregulate the expression of APP, the key enzyme BACE by induce inflammatory cytokines, and then promote Aβ generation, lead to the development of AD. |
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