Relationship Between HBV Genotypes/sub-genotypes Of Hepatitis B Virus And Adefovir Dipivoxil/Entecavir Antiviral Therapy

Objective:To investigate the relationship between the antiviral therapy efficacy of nucleoside analogues and HBV genotypes/sub-genotypes.Methods :The study involved 159 patients with chronic HBV infections, were treated by adefovir dipivoxil(10mg/d) or entecavir(0.5mg/d). The genotypes and subtypes of hepatitis B virus were determined by Nested polymerase chain reaction(nt PCR).The levels of serum HBVDNA replication 、 ALT levels and HBV serologic markers were detected pre or post-treatment 24 weeks 、 48 weeks.The levels of serum HBVDNA replication was tested by Fluorescent quantitative PCR,ALT levels was teated by full-automatic biochemical analyzer, HBV serologic markers were detected by ELISA.Obverse the relationship between the HBV genotypes/sub-genotypes and the antiviral efficacy of adefovir dipivoxil or entecavir treatment.Results:After 24 weeks of adefovir dipivoxil therapy, ALT normalization rate of subgenotype B2 and subgenotype C2 was 66.7% vs 66.2%(x2=0.74,P>0.05),HBe Ag negative conversion rate of two subgenotypes was 27.3% vs 23.1%(x2=0.10,P>0.05), HBVDNA negative conversion rate of two sub genotype B2 and subgenotype C2 was 33.3% vs 30.9%(x2=0.03,P>0.05), respectively.After treatment for 48 weeks, ALT normalization rate of subgenotype B2 and subgenotype C2 was 83.3% vs 82.4%(x2=0.01,P>0.05), HBe Ag negative conversion rate of two subgenotypes was 45.5% vs 34.4%(x2=0.49,P>0.05),HBVDNA negative conversion rate of two subgenotypes was 58.3% vs 48.5%(x2=0.39,P>0.05). After 24 weeks of entecavir therapy, ALT normalization rate of subgenotype B2 and subgenotype C2 was 71.4% vs 69.6%(x2=0.02,P>0.05), HBe Ag negative conversion rate of two subgenotypes was 33.3% vs 30.8%(x2=0.03,P>0.05),HBVDNA negative conversion rate of two subgenotypes was 42.9% vs 39.3%(x2=0.06,P>0.05), respectively.After treatment for 48 weeks,ALT normalization rate of subgenotype B2 and subgenotype C2 was 85.7% vs 83.9%(x2=0.03,P>0.05), HBe Ag negative conversion rate of two subgenotypes was 50% vs 44.9%(x2=0.10,P>0.05),HBVDNA negative conversion rate of two subgenotypes was 71.4%vs 67.8%(x2=0.07,P>0.05).Couclusions:The study showed that genotype C(C2) is a predominant HBV genotype among people with chronic HBV infections in Shanxi province.HBV subgenotypes B2 and C2 have no significant difference in virologic, biochemical, and immunologic response to ADV or ETV.