Clinical Proteomics Was Applied In Researching Diabetic Nephropathy

Objects: Up to date the pathogenesis and predicting the progress of diabetic nephropathy(DN) are still unclear. Meanwhile the early diagnosis biomarkers of DN are lack. Clinical proteomics was applied in this study to explore molecule involving into the pathogenesis of DN and to investigate biomarkers of early diagnosis and predicting the progression of DN. Methods: Four groups of age-matched and gender-matched subjects were enrolled in this study. Group A is the normal controls. Group B,C,D are type 2 diabetes patients whose urinary albumin creatinine ratio(ACR) is less than 30mg/g creatinine, 30mg/g creatinine to 300mg/g creatinine, and more than 300mg/g creatinine respectively. The fasting plasma was collected and a plasma pool was formed in each group. After detaching albumin, four groups of plasma with equivalent protein were separated with two-dimensional electrophoresis(2DE), then were stained with silver staining kit. The experiment was repeated three times and the data were analyzed with specified software in Image Master 2D Platinum(GE company). The proteins which expressed significantly differently among four groups were determined and then detected with Matrix assisted laser desorption/inionation time of fight mass spectrometry(MALDI-TOF MS). Results: A total of 2119 protein spots were detected and matched after separation with 2DE among four groups. Eleven pots were dug and four of them were successfully determined with MS. The expression of mannan-binding lectin serine protease 2(MASP2) elevated significantly in group C. Prothrombin expressed much higher in group D. Meanwhile, the expression of hemoglobin subunit alpha and Ig alpha-1 chain C region increased significantly in group D. Conclusions: Clinical proteomics is one of valuable and efficient tool in clinical practice and research of DN. Mannan-binding lectin serine protease 2 may participate into the pathogenesis or progress of diabetic nephropathy in type 2 diabetic patients. Activation of coagulation may play a role in the progress of DN. hemoglobin subunit alpha and Ig alpha-1 chain C region might associcate with the end-stage renal disease in DN.