Concentration Analysis Of Vascular Endothelial Growth Factor And Connective Tissue Growth Factor In The Vitreous And Serum With Proliferative Diabetic Retinopathy

Objective: To determine the concentrations of serum and vitreous vascular endothelial growth factor(VEGF) and connective tissue growth factor(CTGF) in patients with Proliferative Diabetic Retinopathy(PDR) and Idiopathic Macular Hole(IMH), and investigate the relationship among VEGF, CTGF and PDR.Methods: Vitreous and serum samples were collected from 20 PDR patients and 6 IMH patients. Enzyme-linked immunosorbent assay(ELISA) was used to determine the levels of VEGF and CTGF.Results: The concentrations of VEGF and CTGF in both serum and vitreous with PDR were significantly higher than the control group. As the progression of PDR course, the concentrations of VEGF in vitreous decreased firstly and then increased. The vitreous VEGF levels in the stage of V was significantly lower than those in the stage of VI. There was no significant difference of vitreous VEGF levels among other stages. In addition, there were no significant difference of serum VEGF levels, serum CTGF levels,vitreous CTGF levels and the ratio of CTGF and log10 VEGF among the three stages.Vitreous CTGF levels was positively correlated with serum CTGF levels in PDR patients. There was no significant correlation between serum VEGF levels and serum CTGF levels, vitreous VEGF levels and vitreous CTGF levels, serum VEGF levels and vitreous VEGF levels. There was no significant associations between serum and vitreous VEGF, CTGF concentrations and age, duration, Hb A1 c.Conclusion: The serum and vitreous VEGF and CTGF were closely related to the occurrence and development of DR. Peripheral blood CTGF concentrations may be helpful to prompt the vitreous CTGF concentrations.With the progression of PDR course, the degree of angiogenesis may relief firstly and then aggravate.