Expression Of CDC2 And PFTK1 Protein In Esophageal Squamous Cell Carcinoma And Their Significance

Objective:To investigate the expression of CDC2 and PFTK1 in esophageal squamous cell carcinoma and to analyze their relationship with biological characteristics of malignant, clinical stage, pathological grade and invasion and metastasis of esophageal squamous cell carcinoma and clinical significance.Methods: 60 primary esophageal squamous cell carcinoma tissue specimens and para-cancerous tissues were obtained from the First Affiliated Hospital of Henan University of Science and Technology. They were diagnosed and surgically treated form March 2014 to June 2014, including 20 cases of male, 40 cases of females, age ranged 45-78 years old with a median age of 61 years old. Clinical staging was defined according to UICC published in 2009 PTNM staging criteria, 8 cases of stage I, 24 cases of stage II, 28 cases of stage III. Pathological grading included 14 cases of high differentiation, 36 cases of middle differentiation, 10 cases of low differentiation, which occurred lymph node metastasis accounted for 28 cases. Using immunohistochemical SP method to detect the the expression of CDC2 and PFTK1 in esophageal squamous cell carcinoma.Results:1.The positive expression of CDC2 protein presented brown yellow particles,was mainly located in the cytoplasm, sometimes also expressed little in the nucleus. Positive rate of CDC2 expression in 60 cases of esophageal squamous cell carcinoma was elevated, whereas it was low expression in para-cancerous tissues. The positive expression rates in esophageal squamous cell carcinoma, para-cancerous tissues varied significantly(P<0.05). 2.The expression of CDC2 protein were significantly correlated with depth of tumor invasion, lymph node metastasis and clinical stages(P<0.05), but not correlated with gender, age, degree of differentiation(P>0.05). 3. The positive expression of PFTK1 protein presented brown yellow particles,was mainly located in the cytoplasm. Positive rate of PFTK1 expression in 60 cases of esophageal squamous cell carcinoma specimens was elevated, whereas it was low expression in para-cancerous tissues. The positive expression rates in esophageal squamous cell carcinoma, para-cancerous tissues varied significantly(P<0.05). 4. The expression of PFTK1 protein were significantly correlated with depth of tumor invasion, lymph node metastasis and clinical stages(P<0.05), but not correlated with gender, age, degree of differentiation(P>0.05).5.The expression of CDC2 and PFTK1 were significantly positively correlated(r=0.367, P=0.004)(P<0.05).Conclusion: 1. The positive expression of CDC2 was mainly located in cytoplasm, few nuclear expression, The positive expression of PFTK1 was mainly located in cytoplasm, all presenting a regional or diffuse distribution.2. The expression rates of CDC2 and PFTK1 protein in cancer tissues were higher than those in para-cancerous tissues, suggesting the up-regulated expression of CDC2 and PFTK1 may be associated with the occurrence of esophageal squamous cell carcinoma.3. The positive rates of CDC2 and PFTK1 in esophageal squamous cell correlated with clinical stages, depth of tumor invasion and lymph node metastasis, but not correlated with gender, age, degree of differentiation(P>0.05). Show that both might be associated with esophageal squamous cell carcinoma invasion and metastasis4. The positive rates expression of CDC2 and PFTK1 in esophageal squamous cell carcinoma were positively correlated, suggesting that they may participate in the development of esophageal squamous cell carcinoma.