The Expression And Significance Of Col1al And Col3al In Patients With Liver Echinococcus Multilocularis Infected

Objective:To investigate the expression of Col1 al and Col3 al in liver tissue of patients with alveolar hydatid infection.Methods : 1. Subjects: Select 20 patients with liver alveolar hydatid in Hepatopancreatobiliary Surgery Department, Affiliated Hospital of Qinghai University. 2.(1) Collect the pathological specimens of operation patients with liver alveolar hydatid, the pathological specimens were divided into group A : tissue around the lesion(according to the lesion edge 2cm); group B: normal tissue(more than 2cm from the edge of the lesion). Two groups of specimens preserved in-80 ℃ refrigerator to spare.(2)The pathological specimens were sent to the pathology department of our hospital and made into paraffin section which 3um thick.(3)HE and Masson staining, Observe the pathological changes and fibrosis of tissue around the lesion with Echinococcus multilocularis infection under the light microscopy; Detect the expression and localization of Col1 al and Col3 al in liver tissue by immunohistochemical.(4) Disposal data and perform a statistical analysis, then draw the conclusion.Results:The fibrosis score of adjacent normal tissue and tumor tissue was: T = 132, P <0.01, they were statistically significant. The CRI of Col1 al and Col3 al in the tissue around the lesion and normal liver tissue respectively was(7.45 ± 1.85 VS 3.10 ± 1.02),(8.00 ± 1.62 VS 3.5 ± 0.89), t values respectively was-9.21,-10.88, the difference had significant statistical significance(p <0.001).Conclusion:There were hepatic fibrosis in tissue around the lesion of patients with alveolar hydatid infection; The main components of collagen were Col1 al and Col3 al in tissue around the lesion.