Studies On The Proteome Of Echinococcus Multilocularis Protoscolece

The proteins of Europe Echinococcus multilocularis protoscolece were analyzed by two-dimension electrophoresis (2-DE) separating technique, Western-blotting, Mass spectrometry (MS) technique, and protein bioinformatics. After analyzed 2-DE pattern by ImageMaster software, about 485±18 protein spots were acquired, their molecular weight were about 10-120kDa and the isoelectric points were about 3.5-9. Then 90 protein spots rich in content were chosen and identified by MS to acquire their Peptide Mass Fingerprint(PMF). After analyzed by the software of Mascot, some kinds of proteins highly related to the growth ,development, movement and regulation of the parasite were aquired, which included: actin, transketolase, thioredoxin peroxidase, glutathione transferase, HSP70, HSP20, actin-binding and severin family group-like protein, paramyosin, microtubulin, 78kD glucose regulated protein, tropomyosin, 14-3-3 protein, Caspase-3, hydatid disease diagnostic antigen P-29, and etc. Result from the analysis of antigens expression of Europe Echinococcus multilocularis protoscolece showed, cytoskeleton proteins and chaperonins are the main antigens referring to the host—parasite interaction. Further studies on this field will not only offer some clues about the host—parasite interaction mechanism, but all these relative reseaches will become the bases of finding efficient vaccine candidates and drug targets.What's more, the protein and antigen patterns of Europe Echinococcus multilocularis were also contrasted with those of Echinococcus sibiricensis. As a result, there were only minor differences in protein expression level, but some obvious differences between the antigen patterns of these two types of Echinococcus are found, for example, there is a differential 124kDa antigenic band in Europe Echinococcus multilocularis. While the HSP70 and paramyosin antigens of Echinococcus sibiricensis induce stronger immune response than the former. All these results show that the two types of echinococcus are immunologically different.