Studies On The Chemical Constituents Of Acanthopanax Calyx Hanging Brancheshook Stem And Hypoglycemic Active Site Selection

Rubus alexeterium Focke belongs to the Rosaceae rubus, and there was no relevant reports at home and abroad about Rubus alexeterium Focke. This paper focuses on the research of chemical composition 、 the screening of the different extraction parts of hypoglycemic activity and total flavonoids content study of the branch from Rubus alexeterium Focke for the first time, and preliminaried studied of its mechanism, which provided the reference basis for the research of new diabetes drugs.By retrieving and summarizing lots of literatures, this paper reviewed the Rubus and Rubus alexeterium Focke main chemical components and pharmacological activity, providing a reference basis for further study on Rubus alexeterium Focke.This thesis adopted different polar solvent to percolate extraction Rubus alexeterium Focke branch, and had been extracted with petroleum ether、ethyl acetate extracts、95% ethanol extract、70% ethanol extract and 30% ethanol extracts. Adopting ultrasonic extraction to extract the Rubus alexeterium Focke branch residue, got water extracted part. Respectively using macroporous adsorption resin and polyamide to adsorb water extraction, got 6 extraction sites. The paper groped for the best separation conditions by high speed counter current chromatography in ethyl acetate extract and 95% ethanol extract. The optimum conditions for separation of ethyl acetate part of high speed countercurrent chromatography was n-hexane: ethyl acetate: methanol: water =5:4:3.5:1,and the optimum conditions for separation of 95% ethanol extract of high speed countercurrent chromatography was chloroform: n-butanol: methanol: water =4:0.5:3:2.This paper studyed the intervention effect of Rubus alexeterium Focke branch with petroleum ether、ethyl acetate extract、95% ethanol extract、70% ethanol extract and 30% ethanol, on the part of KKAy diabetic mice body weight、fasting blood sugar、diet and water intake、insulin tolerance and the biochemical indicators. Through the comprehensive statistical we knowed that the ethyl acetate extract and 30% ethanol extract had a good effect. Ethyl acetate extracts could cause the decrease of body weight of mice. Compared with model group, the ethyl acetate extract had significant difference on mice body weight and kidney ratio. The ethyl acetate extract and 30% ethanol extract could make mice diet and water intake and the amount of the fasting blood glucose decreased. At the same time, ethyl acetate extract and 30% ethanol extract could make the biochemical indicators of TG, TC and LDL content decrease, and HDL content increase.Using the method of silica gel column chromatography 、 Sephadex and semi preparative HPLC coupled to separate and purify the ethyl acetate extract, got seven compounds. Finally five compounds had been identified, they respectively were dibutyl phthalate 、bergeninum、β-sitosterol、3,4-dihydroxy benzoic acid(Protocatechuic acid)、ursolic acid. There was two kinds of three terpenes which was not identified was three terpenoids, which can be studied pharmacological activities and mechanism for the further.Using Realtime PCR method to study the influence of the ethyl acetate extract on the amount of five gene of muscle tissue in mice with KKAy PPAR alpha. The ethyl acetate extract could make the muscle tissue of mice pparα mRAN and PPARγ mRAN two gene expression increase, and make the muscle tissue of mouse glut4 mRAN、mnsod mRAN and ampk1 mRAN three gene expression decrease.This paper usd UV spectrophotometry to study the total flavonoids content of Rubus alexeterium Focke branch, and investigated the effects of ethanol concentration、 ratio of material to liquid ratio、 ultrasonic time and ultrasonic frequency on total flavonoids in plant extract. By orthogonal test and the idea of saving energy comprehensive consideration, determine the optimal process conditions of Rubus alexeterium Focke branch total flavone extraction was ethanol concentration 50%, ratio of solid to liquid 1:40, ultrasonic power 100 W, ultrasonic time 60 min, ultrasonic frequency 2 times. The innovation of this paper:The study of Rubus alexeterium Focke branch was the first time.