Association Between Gene Polymorphism Of Chemokine CCL2 And Bladder Urothelial Carcinoma

Objective To investigate the correlation of gene polymorphism of chemokine CCL2-2518T> C and bladder urothelial carcinoma.Methods The genotype and gene frequencies of CCL2-2518T>C polymorphisms were tested respectively using q PCR-HRM method and Taq Man probe method in 85 bladder urothelial cancer samples and 100 healthy samples to validate the reliability of CCL2-2518T>C polymorphic analysis in q PCR-HRM method, to analyze the differences between healthy people and urothelial carcinoma patients in CCL2 genotype and allele frequency distribution, and to analyze the differences in different grades and phases of bladder urothelial patients in genotype and allele frequencies of CCL2.Results 1. Genotyping results between qPCR-HRM method and Taqman probe method were complete concordance.The genotype distribution of bladder urothelial cancer group was: TT type 13(15.3%)、TC type 39(45.9%)、CC type 33(38.8%). Its allele frequency distribution was: T allele 65(38.2%)、C allele 105(61.8%).The genotype distribution of normal control group was: TT type 12(12.0%)、TC type 50(50.0%)、CC type 38(38.0%),The allele frequency distribution was: T allele 74(37.0%)、C allele 126(63.0%)。2.There were no statistical differences between bladder urothelial cancer group and normal control group in CCL2-2518 T>C genotype and allele distribution. p were 0.764 and 0.807 respectively.3.The bladder urothelial cancer patients in different sex, different ages and different smoking histories had no statistical difference in genotype and allele distribution of CCL2-2518T>C. p of them were all greater than 0.05.4. There were statistical differences in high grade and low grade of bladder urothelial cancer patients in genotype distribution of CCL2-2518T>C(p=0.041)and there were no statistical differences in allele(p=0.069).We also found that relative risk of high grade bladder urothelial cancer in people carrying CC genes was 3.137 times in people carrying TT+TC genes(p=0.015,95%CI 1.026-8.025).5. There were statistical differences between non-muscle invasive bladder cancer and muscle invasive bladder cancer in genotype distribution of CCL2-2518T>C,(p=0.034). There were no statistical differences in allele(p=0.084); The relative risk of muscle invasive bladder cancer in people carrying CC genes was 4.5 times in people carrying TT+TC genes(p=0.015,95%CI 1.257-16.115).Conclusion 1.The q PCR-HRM method was reliable to analyze CCL2-2518T>C.2. There were no statistical differences between bladder urothelial cancer group and normal control group in CCL2-2518 T>C genotype and allele distribution. However there were statistical differences in different grades and different phases of bladder urothelial cancer patients in genotype distribution. There were no statistical differences in allele distribution. The relative risk of high grade bladder urothelial cancer and muscle invasive bladder cancer in people carrying CC genes was significantly higher than others. Therefore there was a certain degree of correlation between CC genotype at CCL2-2518T>C sites and bladder urothelial cancer differentiation and its infiltration degree.