Research Of XPD Gene Suppress Proliferation And Migration In Human Hepatocellur Carcinoma HepG2 Cell As Well As Dysregulate Autophagy In Vitro

Objective: To investigate the effect of XPD on the biological function of hepatocellular carcinoma cell HepG2, including cell proliferation, apoptosis,migration as well as autophagy.Methods: The recombinant plasmid pEGFP-N2 / XPD and empty vector pEGFP-N2 were transfected into human hepatoma cell line HepG2 through liposome transiently.Experiment was divided into four group{1) control group, 2) liposome group, 3) pEGFP-N2 group, 4) pEGFP-N2 / XPD group}. The expression of green fluorescent protein reporter gene was detected by fluorescence microscopy, MTT was used to observe the proliferation of tumor cells, apoptosis was analysed by flow cytometry, colony formation experiment was used to detect the clonogenic capacity of the HepG2 cells in vitro,scratch healing experiment and transwell assay were used to detect the migration capability of the HepG2 cells. Western blotting was used to measured the protein expression level of XPD and autophagy marker LC3,P62.Results: The HepG2 cells which transfected with empty vector pEGFP-N2 and recombinant plasmid pEGFP-N2 / XPD cells emitted green fluorescence under the fluorescence microscope,indicating that the transfection efficiency is relatively high.As compared with the control group, the XPD overexpression group showed increased apoptosis rate(p<0.01), decreased cell proliferation(p<0.01), colony formation(p<0.01) and migration capability(p<0.05), and significantly increased intracellular expression levels of LC3(p<0.01),while significantly decreased the expression levels of P62(p<0.01).Conclusion: Overexpression of XPD gene can induce apoptosis and inhibit proliferation, colony formation and migration of hepatoma cells. as well as dsyregulate intracellular autophagy level.