Phytochemical Investigation On The Diterpenes From The Roots Of Daphne Genkwa And Anthocyanins From The Fruits Of Lycium Ruthenicum Murr

The dissertation described phytochemical investigation on diterpenes from the roots of Daphne genkwa, and anthocyanins from the fruits of Lycium ruthenicum Murr.Daphne genkwa belongs to the genus of Daphne of the family Thymelaeaceae, a traditional Chinese medicine(TMC) for the treatment of dispelling retained water, abortion, and mammary cancer. Pharmacological research showed daphnane-type diterpenes from D. genkwa had prominent anticancer and anti-HIV activities. The fresh roots of D. genkwa were extracted with methanol by reflux. 21 daphnane-type diterpenes were isolated from the CHCl3 fraction, which included 6 new ones(1-6) and 15 known ones(7-21).Lycium ruthenicum Murr. belongs to the genus of Lycium of the family Solanacea, and has been found to contain the highest proportion of anthocyanins. Anthocyanins is a potent antioxidant, recent research showed that anthocyanins have various biological activities such as antioxidant, anti-cardiovascular, anti-diabetes, hepatoprotective, anti-tumor etc. In this research, the fruits of L. ruthenicum was extracted by acid water, 5 different anthocyanins, along with 5 other kind of compounds, including one new compound, were isolated from the extract.The structures of the isolated compounds were elucidated by means of integrated analysis of spectral data, including MS(LR- and HR-MS), IR, UV, optical rotations, 1D(1H and 13C) and 2D(HMQC, HMBC, and NEOSY) NMR technologies, and comparison with those reported in literatures.The daphnane-type diterpenes isolated were elvaluated for the antiproliferation effect angainst human cancer cell lines DU145 and A549. On the whole, stronger inhibiton against DU145 cell than against A549 cell were obsereved in daphnane-type diterpenes, among which compound 9, 20 and 21 exhibited strong selective inhibiton against DU145, with the IC50 below 1 μM.