Synergism Of Glucosamine Sulfate In Relieving Effect Of Hyaluronic Acid By Oral Administration On The Arthritis

Objective: Arthritis is the common chronic disease in the life. There are many people of different ages who have the arthritis, the number is rising gradually. The chronic disease has the negative effect on people’s daily life. Many patients select the intra-articular injection of HA to cure the arthritis, but the method brings patients huge pain in order that most patients can’t bear the pain and have to give up the therapy. Hyaluronic acid and glucosamine sulfate are the natural products,which are the ingredients in the joint.As the functional food, eating value has been widely accepted by the public. According to the report, to research the effect of HA and GS of oral adminstration on the arthritis from 3 aspects:(1) After adding GS into HA, to detect the charcter of HA complex liquid.(2) Study on the Synergism of Glucosamine Sulfate in Relieving Effect of Hyaluronic Acid by Oral Administration on Mouse with Adjuvant Arthritis.(3) According to comparsion of HA complex liquid of 3 concertration, to research the effectiveness of HA+GS(2:1).Methods: According to the research, to design the method of experiment:(1) Taking the HA as the standard, to detect the pH, moisture content, viscosity and glucuronic acid content. In order to observe whether the charcter of HA is influenced.(2) 50 8-weeks-old ICR male mice are devided into 5 group at random: normal, model,HA, GS, HA+GS. Except normal group, animal models with adjuvant arthritis of other4 groups are established through injecting 0.1ml freund’s complete adjuvant(FCA) into left rear feet, according to treatment of HA and GS by oral administration for 4 weeks,the weight, thickness, width and swelling degree of mice rear feet and ankle are observed respectively. The concentration of IL-1 and TNF-α in the serum of mice are detected by means of enzymelinked immunosorbent assay(ELISA).(3) 50 8-weeks-old ICR male mice are devided into 5 group at random: normal,model,HA+GS(2:1), HA+GS(1:2), HA+GS(1:1). Except normal group, animal models with adjuvant arthritis of other 4 groups are established through injecting 0.1ml freund’s complete adjuvant(FCA) into left rear feet, continuing to raise mice for 10 days, to taking tail blood, and then oral administration for 30 days. During the period of(4) experiment, to measure the feet thickness and width and to take blood. In the end,to detect the concertration of IL-1 concertration before and after the oral administration by means of enzymelinked immunosorbent assay(ELISA).Results:(1) The detection of HA and HA complex liquid about index: pH 6.30 and 6.09;moisture content 5.47% and 5.84%; viscosity 209.59 and 209.91; glucuronic acid content 49.73% and 48.23%.(2) Swelling degree of mice feet and ankle can be decreased by HA and GS by oral administration, and detumescence effect of HA+GS is better, which lead to obvious decrease of concentration of IL-1 and TNF-α in serum.(3) According to the analysis for swelling degree of mice feet, we found that swelling degree of feet and IL-1 concertration had the obvious decreasing tendency after 3kinds of HA complex liquid. There is the significant difference among 3 groups and model group(P<0.05). Among 3 groups, the effect of HA+GS(2:1) is better than HA+GS(1:1), there is the significant difference between 2 groups(P<0.05).Concertration:(1) After adding GS into HA, the charcter of HA hasn’t changed. The measurement result of HA complex liquid accord with the national regulation.(2) Compared with HA and GS, adding GS in HA to offer mice by oral administration,which can release the swelling symptom of mice feet more effectively and rapidly.It can also decrease the concentration of IL-1 and TNF-α in the serum, which have a better curative effect on mice arthritis.(3) The oral administration of HA complex liquid of 3 concertration can decrease the swelling degree of mice feet and IL-1 concertration, and have positive effect on the arthritis. There is the insignificant difference among effect of HA complex liquid of 3concertration. Relieving effect of HA+GS(2:1) is better than that of HA+GS(1:1)obviously, but there is the insignificant difference between HA+GS(2:1) and HA+GS(1:2).