The Correlative Study Of DTI And Pathology In Radiation-induced Brain Injury

Objective To research the change of Magnetic resonance diffusion tensor imaging(DTI)and pathology of radiation-induced brain injury and to investigate the apply value to early radiation-induced brain injury with DTI.Material and Methods 30 rats were divided into 2 groups randomly: blank control and irradiation groups. The irradiation group rats were performed with MRI, DTI, HE staining,immunohistochemical staining of MBP and NF,electron microscopy on 1, 3, 6,9, 12 months after radiation. Measure the FA. The absorbance of MBP and NF in immunohistochemical staining was measured,the changes of myelin and neurofilament were observed in electron microscopy and the changes of vascular were in observed HE staining.Results 1、The differences of FA decreased gradually after irradiation, with the time went on, FA decreased more obviously, the differences of FA between any radiation groups and control group were statistical significant.2、The differences of MBP absorbance decreased gradually after irradiation, with the time went on, MBP absorbance decreased more obviously, The differences of MBP absorbance between any radiation groups and control group were statistical significant.The differences of NF absorbance decreased gradually after irradiation, with the time went on, NF absorbance decreased more obviously, The differences of NF absorbance between any radiation groups in one months after radiation and control group were statistical significant.3、The FA positively correlated with the MBP(r=0.942). The FA negatively correlated with NF(r=-0.924).Conclusion DTI can reflect the pathological changes of the white matter in radiation-induced brain injury at a micro level.