Effects Of Chk1 Gene Expression On Sperm Concentration And Vitality

Objective:By detecting different density and vitality of sperm DFI(DNA fragmentation rate, DNA fragmentation rate) sperm cell viability and associated expression, Chk1 gene density and the relationship between research activity and Chk1 gene expression in sperm cells, and to further explore Chk1 gene and the correlation between male infertility. Methods:The collected specimens of human semen routine examination of semen, according to the density and vitality were divided into normal control group, less refined group, weak sperm group and less weak sperm groups of samples each of 20 parts. Using acridine orange method(AOT) is detected in four groups of sperm DNA fragmentation rate, using eosin staining-Y 4 groups of sperm viability, using RT-PCR method to detect the sperm Chk1 m RNA expression, and calculate the relative expression using western-blot method to detect the expression of each group Chk1 protein in sperm, and calculate the relative expression. Results:1) Acridine orange assay DNA fragmentation rate found that, compared with the normal group, group less sperm, weak sperm group, oligoasthenospermia genomic DNA fragmentation was no significant difference(P> 0.05);Eosin-Y sperm viability staining found that, compared with the normal group, low sperm group, weak sperm group, oligoasthenospermia group significantly reduced sperm viability.2) The value of each group divided according to DFI% DFI> 30% and DFI≤30%,so there were differences between the groups(P<0.05),and the sperm motility, sperm concentration and sperm viability were statistically significant between(P <0. 05).3) RT-PCR analysis showed that Chk1 gene was expressed in normal control group and the other three groups of sperm, among the four groups relative expression of Chk1 gene group differences were significant(P <0.01). The relative expression level with sperm density and viability were Pearson correlation analysis showed that the relative expression of the density and vitality of sperm Chk1 gene correlated(P <0.05).4) Western-Blot test showed that Chk1 protein in normal control group and the other three groups of sperm were expressed relative expression between the two groups of four groups of Chk1 protein significant difference(P <0.01). Conclusions:1) DFI and sperm concentration,vitality have no significant correlation, but DFI abnormalities may be important factors affecting sperm concentration and vitality.2) It is closely related to sperm viability and sperm density, vitality.3) Chk1 significant in human sperm expression, and in normal men with low sperm was significantly different from weak sperm and fewer patients with weak sperm expression, the expression of which changes may affect the density and vitality of sperm cells.