| 【Objective】1. To observe the common condition and living condition of the postoperative rats by establishing a simple, high success rate and stable rat partial portal vein arterialization model.2. To explore the effects of partial portal vein arterialization combined liver failure rat model on liver functions and the pathological changes.【Methods】Experiment one: Establishment and observation of rat model1. Partial portal vein arterialization model: Using the allograft materials(on the lower end of the sleeve cuff, side end exclusion)and setting into the suture cuff to establish the model. Resect the left kidney, and the left renal artery were connected with portal vein, superior mesenteric vein stump by allogeneic blood vessel materials.After the model established, D-galactosamine was intraperitoneally administered at the dose of 1300mg/kg one time.2. Liver failure model: To explore left kidney by separate tissues, cut ureter and left renal At V, and then ligate it after stripping renal fascia. Free the tissue surrounding portal vein to 1,2 branches, occlude the portal vein for 16 min.D-galactosamine was intraperitoneally administered at the dose of 1300mg/kg one time.3. Control group model: To ablate the left kidney, occlude the portal vein for16 min. 0.9% physiological saline was intraperitoneally administered at the dose of1300mg/kg one time.4. Observing: 70 SD rats were randomized to divide into three groups: PPVA,LF and control group. To observe the operation time, portal vein occludes time and rats’ common condition, living condition. As well as hepatic tissue morphology,pathological changes.Experiment two: The effects of partial portal vein arterialization combined liver failure rat model on liver functions130 SD rats were randomized to divide into three groups: PPVA(Hepatic failure plus PVA, n=50),LF(Hepatic failure plus the left nephrectomy, n=50),co ntrol group(left nephrectomy, n=30). 5 rats in each group were sacrificed to get samples 12 h,24h,36 h,48h,72 h after operation.Observe the serum level of glutamic-oxalacetic transaminease(AST),alanineaminotranferase(ALT), totalbilrub in(TBil), albumin(ALB), IL-6, TNF-a, LPS.【Results】Experiment one: Establishment and observation of rat model1. PPVA and liver failure groups were successfully established. The former’s success rate is 96.7%,operation time is(55.93±6.56)min, blocking time is(16.7±1.88)min. The latter’s success rate is 100%(30/30),average operation time is(32.5±2.81)min, blocking time is(16±0)min.2. The rats in control group had flexible movement, clean fur and good eating.18 hours after the model established, the rats in PPVA and liver failure groups’ sprits were drooping, the body was curled up. Their food intake and weight declined and pain reaction was unresponsive or disappear. Urine yellow, oliguria. Part of the rats had the nervous excitement symptoms, the whole body twitch. A little part of the rats were vomiting.3. The 9 rats in PPVA group were died within 72 h after modeling. The survival rate was 70%.One died during the operation and others died after operations. The 14 rats in LF group died within 72 h, which at a survival rate of 53.3%.All of them died after operation. The survival rate of control group was 100%.4. Observation of paraffin sections : Control group, the structure of liver lobularand lobular central vein was complete and clear. LF group, liver lobular structure were damaged and a large number of hepatic cells were necrosis. Liver cable and hepatic sinus structure was fuzzy. There were inflammatory cells infiltration in portal area and central vein. PPVA group, hepatic cells arranged in disorder. There were a large number of inflammatory cells infiltration. Hepatic sinus bleeding and necrosis.Experiment two: The effects of partial portal vein arterialization combined liver failure rat model on liver functions1. The serum levels of AST、ALT、TBIL in model PPVA and LF increased at12 h after the model established and reached a peak at 48 h.And then, began decline.Compared to LF group, the serum levels of AST、ALT in PPVA group were lower in5 time points, which was statistically significant difference(P<0.05).And the serum level of TBIL at 24 h,36h,48 h,72h after the model established was statistically significant difference(P<0.05).The serum level of ALB at 36 h,48h,72 h was lower than control group, which was statistically significant difference( P <0.05).Compared with LF group, the differences in PPVA group was not statistically significant(P>0.05).2. The serum levels of LPS、IL-6 of control group in any time point had no changes after the model established, but increasing at 12 h in the PPVA group and LF group. Reached a peak at 72 h, which was statistically significant difference(P<0.05).The serum levels in PPVA group were higher than control group, which was statistically significant difference(P<0.05).The serum level of TNF-αhas no change within 72 h,but lower than PPVA group, which was statistically significant difference(P<0.05). The serum levels in PPVA group and LF group reached the peak in 12 h,and then declined, which was statistically significant difference(P<0.05).【Conclusions】1. The rat model of portal vein arterializations, which established by the suture cuff technique was stable. The operation is easier than traditional operation, and hasa high success rate, low immunological rejection rate. Which lead to a ideality animal model in clinical study about portal vein arterializations.2. The rats acute liver failure model induced by D-galactosamine intraperi toneally administered could simulate the pathological and functional changes i n liverfailure patients. It has the advantages such as operation easily, quickly,efficiently,stable and reliable.3. Part portal vein arterializations could alleviate the pathological changes in acute liver failure model induced by D-galactosamine. At the same time, it can also improve the liver functions by increase the blood supply, slow the progress of liver failure, decrease the level of LPS in rats, improve detoxification functions of liver as well as decrease the release of IL-6、TNF-α. |
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