The Influence Of Gap Junctional Intercellular Communication Between Quinine, Carbenoxolone Micro Arterial Smooth Musclecells Of Guinea Pigs

Objective: This study compared quinine, affecting carbenoxolone on guinea pig mesenteric artery fine micro gap junctions between muscle function and expression, and to explore changes in connexin micro mesenteric arteries in the guinea pig in. Methods: Select guinea 18, were randomly divided into normal group, the intervention group and quinine carbenoxolone intervention groups of six; select SD rats 24. Acutely isolated guinea pig mesenteric arterioles, using the whole-cell patch-clamp technique investigate the effects of quinine and carbenoxolone on smooth muscle cell membrane capacitance and membrane conductance or resistance on the guinea pig mesenteric artery segments; observe flufenamic acid on the micro-arterial smooth muscle cell membrane capacitance segment film or membrane conductance impact resistance. Results:(1) Smooth muscle cell membrane capacitance and conductance of the superior mesenteric artery segment is about 10 times more digestible single cell, there is a wealth of gap junction channels between prompt cells.(2) Quinine and carbenoxolone can reduce the concentration-dependent segment of superior mesenteric artery smooth muscle cell membrane capacitance and conductance, inhibit cell membrane conductance half inhibitory concentrations were 1.56 and 0.13mmol/L, when quinine greater than or equal 3mmol / L or carbenoxolone greater than equal to 0.3 mmol/L, the superior mesenteric artery segment capacitance, smooth muscle cell membrane conductance values of film or film resistors with a single cell is very close. Tips quinine and carbenoxolone can completely block the gap junctions between cells communications.(3) Smooth muscle membrane capacitance, resistance cell membrane or membrane conductance on flufenamic acid can reduce the concentration-dependent and reversible micro arterial segments.(4) Flufenamic acid suppression mesenteric artery and cerebral artery smooth muscle cell membrane conductanceIC50 56.33 μmol/L, respectively, no significant difference between the arterioles. When flufenamic acid concentrations greater than or equal to 300 μmol/L, film capacitors, membrane conductance or smooth muscle cell membrane resistance mesenteric arteries and cerebral arterioles micro segment and the corresponding single smooth muscle cells are very close. Conclusions: Quinine and carbenoxolone can block the concentration-dependent cell gap between the superior mesenteric artery connecting channels, carbenoxolone stronger inhibitory potency; flufenamic acid can be concentration-dependent and reversible blocking gap junctions between arteriolar smooth muscle cells, flufenamic can be completely blocked when the acid concentration is greater than or equal to 300 μmol/L of the gap between micro arterial smooth muscle cells connected; flufenamic acid on gap junction between the superior mesenteric artery and cerebral arterioles cells homology.