Research On Expression Of Phosphorylation Connexin 43 In Human Glioma And Correlation With Tumor Cell Proliferation

Objective:Glioma is the most common type of primary intracranial tumor,Glioma 5 years mortality in systemic tumor located in the third. Biological characteristics of human gliomas is extremely complex. In recent years, its surgery, chemotherapy and combined therapy allows patients to be treated in a certain extent, but with the malignant degree of gliomas its recurrence and prognosis were significantly different. With the development of molecular biology and its wide application in the clinical, people gradually realize the tumor and the original the lack of the activation of oncogene and tumor suppressor genes and mutations are closely related. Recent studies show that is closely related to abnormal connexin(Cx) expression with a variety of malignant tumor proliferation and transformation. In this study, operation treatment of glioma samples and internal decompression of normal brain tissue samples as the research object, Cx43 protein expression and phosphorylation of Cx43 protein was detected in the tumor and brain tissue by using Western blot; the classification and grading of glioma WHO tumor cells was determined by using HE staining and immunohistochemistry. Two kinds of proteins in contrast to normal brain tissue and tumor tissues, reseach the glioma cell Cx43 phosphorylation and proliferation of tumor cells relation, preliminary reveal the relationship between phosphorylation of Cx43 and tumor malignant degree, to further explore the clinical value of the treatment of possible. Methods: In my department confirmed operation resection and well preserved human glioma specimens and the corresponding decompression operation of normal brain tissues as the object of study, using HE staining, immunohistochemistry method to determine the nature of grade glioma, proliferation of tumor cells by staining reaction of Ki-67. Western-Blotting technique was used to detect the expression of Cx43 protein and Cx43 protein phosphorylation. Statistical analysis was performed using SPSS19.0 statistical software, measurement data using single factor analysis of variance indicated; comparison between groups using X2 test; differences between ordinal variables using nonparametric rank test; correlation analysis was processed by using Spearman rank correlation analysis. Results:1. In 40 cases the expression of Ki-67 in brain glioma total rate is 85%, the expression of WHOI-IV level in Ki-67 glioma of different grade glioma have significant differences(P<0.001), and showed increasing trend, rank correlation(Spearman) coefficient was 0.837(P<0.001).2. Phosphorylation of Cx43 protein expression was in the WHO I level in glioma mean slightly lower expression in normal brain tissues, but the two no difference(X—=0.3898 VS X— =0.5215, P=0.140), there were significant differences in the expression of other groups. There were significant differences between the groups in the expression of phosphorylated Cx43 protein(P<0.001), showing a gradually increasing trend, with statistical significance.3. Different levels of Ki-67 expression and phosphorylation in brain glioma Cx43 rank correlation(Spearman) coefficient was 0.7699(P<0.001), a close correlation between the two, and there is a positive correlation trend.4. Non phosphorylated Cx43 protein expression than in WHO grade I gliomas in mean slightly higher expression in normal brain tissues, but the two no difference(X— =0.7096 VS X— =0.6914, P>0.05), there were significant differences in the expression of other groups. The expression of non phosphorylated Cx43 protein have significant differences among the groups(P<0.001), decreased, with statistical significance.5. Expression of Ki-67 in gliomas of different grades and non phosphorylated Cx43 rank correlation(Spearman) coefficient of-0.792(P<0.001), a close correlation between the two, and there is a negative correlation trend.6. Different levels of expression of rank correlation of phosphoric acid in brain glioma Cx43 and non phosphorylated Cx43(Spearman) coefficient of-0.8221(P<0.001), a close correlation between the two, and negative correlation trend. Conclusion:1. The higher the pathological grade gliomas, the expression of Ki-67 showed a trend of higher, suggesting that Ki-67 expression can reflect the proliferation potential of glioma.2. Phosphorylation Cx43 protein expression was positively correlated with cell proliferation and tumor tissue pathology classification, The phosphorylation Cx43 protein expression increased with glioma malignant degree of higher, prompt the expresstion of phosphorylation Cx43 protein is closely related to the tumor cell activity and glioma malignant progression.3. Non The phosphorylation Cx43 protein expression and negatively correlated with cell proliferation and tumor tissue pathology classification, The phosphorylation Cx43 protein expression reduced with glioma malignant degree increased, prompt the expression reduce of non phosphorylation Cx43 protein is closely related to the glioma malignant progression, and metastasis.4. Glioma and normal brain tissue containing phosphorylated and non phosphorylated two states of the Cx43 protein, and phosphate with different grade gliomas in phosphorylated Cx43 and non phosphorylated Cx43 expression showed a negative correlation trend.