| Euphorbia lathyris L. is the plants of Euphorbiaceae Euphorbia, has a long cultivated history in our country, widely distributed in south to guangxi, north to jilin, east to zhejiang and west to Tibet, and more than 10 provinces. Euphorbia lathyris L. has a variety of purposes and a high use value, such as a medicinal, ornamental, grease and pesticide plant. Its root, stem and seed can be used as a medicine, in pursuit of water swelling, broken insecticidal, catharsis, sedative and analgesic, anti-inflammatory, antibacterial and antitumor effect. Three extraction methods, including Soxhlet extraction, ultrasonic extraction, and aqueous enzymatic extration, were compared for their extraction efficiency. Based on the single factor experiment, four factors were screened and optimized by using Plackett-Burman (PB) design. Central Composite Design was applied to optimize the selected factors using the seed oil as the response value. The fatty acids were analyzed by gas hromatography-mass spectrometry (GS/MS).To develop an HPLC method for determination of gallic acid,chlorogenic acid,coffee acid,vanillic acid,p-coumaric acid, ferulic acid, rutin, resveratrol, quercetin, kaempferol, baicalein and wogonin in Euphorbia lathyris L. Using HPLC to detection the type and content of 6 kinds of phenolic acids in root, stem, seed and seed coat of Euphorbia lathyris L. By determining removal ability of DPPH free radical, ferric iron reducing antioxidant capacity (FRAP) and peroxyl radical scavenging capacity (PSC) Assay, we evaluated synthetically the antioxidant activity of Euphorbia lathyris L.Through the experiment, we obtained the following results:1. To determine the optimal parameters in extraction of seed oil from Euphorbia Lathyris L. and analyze its fatty acid composition.The effect of extraction solvent, ratio of liquid to dry material, temperature and time on the extraction efficiency of the seed oil were the most obvious. The optimal conditions of extraction were obtained as follows:extraction solvent as petroleum ether, ratio of liquid to material at 30:1, extraction temperature at 90 ℃, extraction time at 9 h. Finally, the fatty acids were analyzed by gas hromatography-mass spectrometry (GS/MS). A total of 8 kinds of fatty acid were identified, accounting for more than 99% of the total fatty acids. The content of unsaturated fatty acid reached more than 90% of total fatty acids, including oleic acid, linoleic acid, a-linolenic acid. Under these conditions the predictive maximum yield of seed oil of Euphorbia Lathyris L was 54.35%, the actual yield was 54.18%, the relative error was 0.12%.2. Using HPLC to detection the type and content of 6 kinds of phenolic acids in root, stem, seed and seed coat of Euphorbia lathyris L. The results showed that six kinds of phenolic acids concentration in 1～300mg/L range with a good linear relationship between peak area, and six kinds of flavonoids concentration in 0.5-300mg/L range with a good linear relationship between peak area The correlation coefficient(R2) is larger than 0.9993. The average recoveries of the six compounds were 91.21%-116.54%, and all RSD values are less than 5.15%. The test results meet the analysis requirement. The method can detect 6 components under the same chromatographic conditions and appears to be simple, accurate, and well reproducible, which could be used for the simultaneous determination of the above-mentioned six compounds in Euphorbia lathyris L.3. The extracts from different parts of Euphorbia Lathyris L. have strong antioxidant capacity. EC50 of eliminating DPPH free radicals by free Phenolic in root was highest (465.35μg/mL), followed by free Phenolic in seed(451.66μg/mL), bound Phenolic in root(325.20μg/mL),bound Phenolic in stem(305.08μg/mL),free Phenolic in stem(246.02μg/mL) and bound Phenolic in seed(123.95μg/mL). The lowest EC50 of eliminating DPPH free radicals was free Phenolic in testa(48.66μg/mL). Similar, reducing capacity of bound Phenolic in seed was highest(1972.43±52.13mg FeSO4/100gDW), followed by free Phenolic in root(1807.50±17.09mg FeSO4/100g DW), free Phenolic in stem(105.71±1.51mg FeSO4/100 g DW), bound Phenolic in stem(71.39±2.95mg FeSO4/100 g DW),free Phenolic in testa(69.29±4.92mg FeSO4/100 g DW) and bound Phenolic in testa(44.86±0.13mg FeSO4/100 g DW). The lowest reducing capacity was bound Phenolic in root(37.69±4.69 mg FeSO4/100 g DW). EC50 of eliminating hydrogen peroxide radicals by free Phenolic in stem was 28.32 mg/mL, and bound Phenolic in stem was 15.08 mg/mL. Antioxidant capacity of free Phenolic in stem was 0.68μmol of Vit. C equiv./100 g DW, and bound Phenolic in stem was 1.25μmol of Vit. C equiv./100gDW. |
PDF Full Text Request