The Expressions Of T Cell Factor 3 In Non-Small Cell Lung Cancer

Bakground:Primary lung cancer is a kind of disease who has a high morbidity and mortality in the world. Among primary lung cancer cases, approximately 80% of the cases are non-small-cell lung cancer. The WNT signaling pathway is one of the basic signaling pathways in the body that directs cell proliferation and differentiation. Recent studies have shown that WNT signaling pathway also plays a key role in the process of the development of tumor. The abnormal activation of the WNT signaling pathways is closely related to the development of lung cancer. When the WNT signaling pathways is abnormal activation, β-catenin migrates to the nucleus, it complexes with members of the T-cell factor (TCF)/Lymphoid enhancer-binding factor (LEF) family of transcription factors. TCF-3 is one of the important members of the TCF/LEF family, but there are very few reports about TCF-3 in lung cancer.Objective:To study the expression and distribution of T cell factor-3 (TCF-3) in non-small cell lung cancer (NSCLC).Methods:(1) TCF-3 expression was examined in 50 non-small cell lung cancer specimens and 50 corresponding normal lung specimens using immunohistochemistry(S-P method). (2) The mRNA level of TCF-3 in 5 NSCLC cell lines (A549, H460, H226, H520, SK-MES-1) and 2 human normal bronchiepithelium cell lines (HBE and BEP-2D) were detected by real-time fluorogenic quantitative PCR. The protein level of TCF-3 gene was detected by Western blotting.Results:(1) Among 50 lung cancer specimens,24 samples showed high expression of TCF-3 (48%), including 22 samples showed TCF-3 expression both in cytoplasm and nuclei, TCF-3 were not detected in 50 corresponding normal lung specimens.(2) The mRNA and protein expression level of TCF-3 was significantly higher in A549, H520, HBE, BEP-2D than its in the sk-mes-1 and H460.Conclusion:There was TCF-3 expression in lung cancer tissue as well as cell lines, TCF-3 expressed both in nuclei and cytoplasm, but mainly in the nuclei.