| Bee venom preparation Shen Fengjing liniment is made up of bee venom as the main raw material, with a variety of bee products and traditional Chinese medicine. In this study, we researched its effect on adjuvant arthritic rats and its preliminary mechanism of Shen Fengjing. So we can provide scientific information and the basis for the clinical treatment on rheumatoid arthritis. The main contents and results were as follows:1. Establish the model of adjuvant arthritic rats. From the aspects of body weight, body hair and reactivity, the related signs indicators about the effect of Shen Fengjing was observed. The results showed that Shen Fengjing could improve the living conditions of adjuvant arthritic rats. Through analyzing the change of body weight, the results showed that the growth of body weight in adjuvant arthritic rats decreased, while Shen Fengjing treatment could significantly increase the growth of body weight(p<0.01).2. Through the measurement of paw swelling degree, we studied the anti-inflammatory effect of Shen Fengjing on adjuvant arthritic rats. The results showed that Shen Fengjing treatment could significantly reduce paw swelling degree of the adjuvant arthritic rats (p<0.01). The maximum paw swelling inhibitory rate was 65.85%.3. Through the determination of organ index of rats, we found that compared with the model group, Shen Fengjing could significantly reduce spleen index and increase thymus index in adjuvant arthritic rats (p<0.01). The results suggested that Shen Fengjing treatment could protect the immune organs of adjuvant arthritic rats.4. We investigated the regulation effect of Shen Fengjing on cytokines levels in serum of adjuvant arthritic rats by ELISA. The results showed that compared with the model group, Shen Fengjing could significantly decrease the contents of TNF-a and IL-1β in all treatment groups (p<0.01), and increase the contents of IL-4 and IL-10 (p<0.01).5. We investigated the regulation effect of Shen Feng)ing on NO and PGE2 levels in adjuvant arthritic rats serum by ELISA. The results showed that, Shen Fengfing therapy could reduce the content of inflammatory mediators NO and PGE2 in adjuvant arthritic rats (p<0.01).6. We used Western Blotting to measure the effect of Shen Fengfing on angiogenesis in adjuvant arthritic rats. The results showed that the treatment of Shen Fengjing had a certain anti-angiogenesis effect. Compared with the model group, the expression of MVD, VEGF obviously decreased in Shen Fengfing 1.0 mL·kg-1 group (p<0.01). It revealed that the Shen Fengjing could decrease the MVD, and reduce the expression of VEGF in order to inhibit angiogenesis.7. In order to research the anti-angiogenesis effect of Shen Fengjing on adjuvant arthritic rats further, we investigated the regulation effect of Shen Fengjing on the content of VEGFR-1, VEGFR-2, MMP-2, MMP-3 and MMP-9 in serum of adjuvant arthritic rats by ELISA. It showed, compared with the model group, Shen Fengfing 1.0 mL-kg-1 group could significantly decrease VEGFR-1 levels (p<0.01), but there was no difference in VEGFR-2 levels of each group; Shen Fengjing treatment could decrease the contents of MMP-2, MMP-3 and MMP-9 in serum of rats(p<0.01).These results preliminary proved that Shen Fengjing in the effective dose range could not only improve the life condition of adjuvant arthritic rats, protect immune organs of rats, but also enhance the immune regulation ability of rats, by reducing pro-inflammatory cytokines TNF-a and IL-1β levels, and increasing anti-inflammatory cytokines IL-4 and IL-10 levels, decreasing inflammatory mediators PGE2 and NO levels, so as to achieve the therapeutic effect. In addition, Shen Fengjing could decrease the content of VEGFR-1, decrease MMP-2, MMP-3 and MMP-9 levels in serum of rats, and reduce the expression of MVD and VEGF, to inhibit rat adjuvant arthritic angiogenesis, so as to achieve the therapeutic effect. |
PDF Full Text Request