The Effects Of CILP2 Overexpression On Metabolism In Vivo

Objective To explore the effects of CILP2 gene on glucose met abolism in mice.Method C57BL/6J mice were randomly divided into six groups:chow diet fed+physiological saline group (NCN group, n=8), chow diet fed+pAd-GFP group (NCG group, n=8), chow diet fed+pAd-CILP2 group (NCC group, n=8), high fat diet fed+physiological saline group (HCN group, n=8), high fat diet fed+pAd-GFP group (HCG group, n=8), high fat diet fed +pAd-CILP2 group (HCC group, n=8). Glucose metabolism, insulin sensitivity were estimated by using intra-peritoneal glucose tolerance test, and hyperinsulinemic-euglycemic clamp studies.Results Compared with HCN and HCG group, the plasma levels of fasting plasma insulin (FIns)、Free fatty acids (FFA)、triglycerides (TG) and total cholesterol (TC) were significantly increased in mice of HCC group (P<0.05 or P<0.01), however, there were no significant differences in the groups fed with chow diet. Over-expression of CILP2 decreased rates of glucose infusion in the process of hyperinsulinemic-euglycemic clamp in mice (P<0.01).The percentage suppression of hepatic glucose production (HGP) in HCC group were 30% and 32% respectively (all P<0.05), much higher than HCN and HCG group.Conclusion Over-expression of CILP2 reduces Lipolysis resistant of insulin, increased insulin resistance of hepatic and peripheral tissue.