MUC1 Cytoplasmic Domain Inhibitor Influence On The Radiosensitivity Of Lung Cancer Cells

Objective:To explore the radiosensitive effect of GO-201 on lung cancer cell line A549 cultured in vitro and its possible mechanism.Methods:MTT assay was used to detecte the growth inhabition rate; the radiosensitive effect was detected by clone formation test. Cell survival cure was fitted by multitarget one-hit model and Dq, D0, SF2, sensitizing enhancing vatio (SER) was calculated. Additionally, flow cytometry was applied to detcet the apoptosis and reactive oxygen species in each group, western blot was conducted to observe the expression of manganese superoxide dismutase (MnSOD) protein.Results:GO-201 could inhibit the proliferation of lung cancer cell; and enhance the radiosensitivity of A549 cells, the SER is 1.14. Flow cytometry analysis obviously increased cell apoptosis and reactive oxygen species in the effect of GO-201 and radiation, the expression of MnSOD protein was decreased in the action of GO-201 and radiation by using western blot (P<0.05).Conclusion:inhibiting MUC1 cytoplasmic domain can improve the radiosensitivity of lung cancer cell by its effect on the level of MnSOD protein.