Expression And Significance Of Adiponectin,Resistin And Apelin In Hepatocyte Steatosis

Objective:To explore the expression and significance of adipocytokines Adiponectin,Resistin and Apelin in hepatocyte steatosis induced by oleic acid.Methods:Human L02 cells were used as controls,L02 cells were induced by oleic acid as model groups which were induced for 24 h,48 h,72 h respectively to establish nonalcoholic hepatocyte steatosis model. Lipid droplets in cells were observed through oil red staining. The levels of triglyceride were measured with analyzed kit.The expression of mRNA and protein of Adiponectin,Resietin and Apelin were measured by qPCR and Western blot.Results:1.Oil red O staining showed that hepatocyte steatosis occurred 24 h after L02 cells were treated with oleic acid and increased greatly at 72 h.2.Triglyceride in hepatocytes of model groups also increased compared with the control group.3.In model groups, the expression levels of Adiponctin mRNA and protein decreased at 24 h and declined remarkably at 72 h,as compared with control groups.4.The expression levels of Resistin mRNA increased at 24 h and elevated remarkably at 72 h in model groups than that in the control group.The expression levels of Resistin protein increased at 48 h and elevated remarkably at 72 h in model groups than that in the control group.5.The expression levels of Apelin mRNA and protein increased at 24 h and elevated remarkably at 72 h in model groups than these in control groups.Conclusion:The expression of Adiponectin is down-regulated while that of Resistin and Apelin are up-regulated in hepatocyte steatosis model,indicating that the regulation of adipocytokines. Adiponectin Resistin and Apelin may be common involved in hepatocyte steatosis.