Studies On The Determintion Of Content And The Characteristic Chromatogram Of Bitter Almond Formula Granules By HPLC

Objective:The bitter almond formula granules were processed by the dry mature seeds of rosaceae wild apricot. It has fall gas cougha and asthma,laxative effect, Often used to treat cough asthma, chest full of phlegm,constipation and dry bowel. Formula particles break through the traditional Chinese medicine yinpian mixed decoction of application forms, avoid Fried clothing, action quick, compounds completely, safety and health, easy to carry,etc. About the quality control method of bitter almond granules, and there is no national standard, also did not see related literature reports. So, in order to control the quality of bitter almond formula granules, we adopted the method of high performance liquid main effective components- laetrile content determination, and set up characteristic fingerprint of bitter almond formula granules, more comprehensive and scientific evaluation of the quality of bitter almond formula granules.Methods:1 The determination content of laetrile in bitter almond formula granulesTake bitter almond recipe particle amount, fine, take about 0.1g,accurately weighed, set 25 ml volumetric flask, add methanol 15 ml ultrasonic treatment(power 250 W, frequency 40 k Hz) 30 minutes, let cool, dilute to the mark with methanol, shake uniform test solution, filtration, the filtrate as.Precision. Precision Weigh amygdalin reference 14.76 mg, set 10 ml volumetric flask, add methanol to dissolve and dilute to volume, shake that was the reference liquor. Take liquor 1ml to 25 ml volumetric flask, diluted with methanol to the mark that was per lml containing amygdalin 59.05μg of the reference solution.Column: Wondasil C18 column(4.6 × 250 mm, 5μm); methanol-0.1%phosphoric acid(5:95) as the mobile phase; detection wavelength of 235 nm.Number of theoretical plates amygdalin peak should not be less than 3,000.2 Study characteristic spectrum of particles of bitter almond formula granulesTake bitter almond recipe particle amount, fine, take about 0.1g,accurately weighed, set 25 ml volumetric flask, add methanol 15 ml ultrasonic treatment(power 250 W, frequency 40 k Hz) 30 minutes, let cool, dilute to the mark with methanol, shake uniform test solution, filtration, the filtrate as.Precision Weigh amygdalin reference 12.05 mg, set 200 ml flask, add water to dissolve and dilute to volume, shake that was per lml containing amygdalin59.05μg of the reference solution.Column: Wondasil C18 column(4.6 × 250 mm, 5μm); mobile phase:acetonitrile-0.1% phosphoric acid(eight ninety-two); detection wavelength:207nm, flow rate: 0.8ml / min, column temperature: 30 ℃. Number of theoretical plates amygdalin peak should not be less than 7000.Results:1 The determination content of laetrile in bitter almond formula granulesThe establishment of a method for the determination of bitter almonds amygdalin recipe particles using high-performance liquid chromatography.Methodological tests showed a good linear relationship within amygdalin injection volume range of 0.1810 ~ 1.1810 μ g regression equation: Y =1.078578 × 106X-4.4407 × 104, r = 0.9998; bitter almond recipe for the test solution particles in 18 h good internal stability; average recoveries sample amygdalin was 98.4%(n = 6). The average content of three batches of bitter almonds measurement of the particle in the formulation of amygdalin 18.46mg/ g.2 Study characteristic spectrum of particles of bitter almond formula granulesThe establishment of a bitter almond recipe particles characteristic fingerprint analysis using HPLC. Methodological tests showed that amygdalin injection volume in the range of 0.20776 ~ 0.62328μg a good linear relationship, the regression equation: Y = 1.188295 × 106 X + 3.6820 × 104, r= 0.9998. The result of 10 batches of bitter almond recipe particle HPLCfingerprints in Import "chromatographic fingerprint similarity evaluation system for the 2008 edition of" evaluation, a total of five peaks selected similarity ten batches of samples were greater than 0.99.Conclusions:This experiment adopts the HPLC method for content of laetrile in bitter almond formula granules and the characteristic fingerprint was studied. On the whole reflects the internal composition similarity between different batches particles, which provide the basis for its quality control.