The Effects Of 25(OH)D3 On Maturation And Antigen-presenting Function Of Dendritic Cells In Tuberculosis

Backgroμd:Tuberculosis threat to human health and safety in the word.Tb and AIDS are the most lethality disease. Tuberculosis is only lower than the rate of AIDS mortality, ranked second in the fatal disease. The World Health Organization report about year 2013 prevalence of tuberculosis, which have 900 million people infected with TB, In 2014 approximately 1.5 million people died from TB. Currently most patients with spinal tuberculosis who facing the resistant of drug, More even, multi-drug resistant tuberculosis, and XDR-TB which make the spine individual chemotherapy facing serious challenges. The chemotherapy of Spinal tuberculosis due to tuberculosis, In order to facilitate the treatment of spinal tuberculosis, we need to depth study of the pathogenesis of tuberculosis.Early in the Europe, the treatment of tuberculosis was rationalized based on its role in the calcification D,.With the rise of anti-TB drugs, 25(OH) D3 used in the treatment of tuberculosis decreased. However, Because of the irregular use of anti tuberculosis drugs and variation of Mycobacterium tuberculosis the problems of tuberculosis drug resistance are gradually emerging, the infection of tuberculosis is harder to treat as normal treatment regimes.25(OH) D3 treatment of tuberculosis reported a lot, but has not reached a consensus view. Some reports said that 25(OH) D3 treat tuberculosis, which not only improve the efficacy of clinical treatment of tuberculosis, but also improve the imaging performance of tuberculosis patients However, some reported that 25(OH) D3 to improve TB patients clinical results did not play a role. Therefore, further study the role of 25(OH) D3 in tuberculosis in need.Innate immunity of the host that 25(OH) D3 plays a crucial role in the immune process in tuberculosis. Recently, there is literature to clarify the 25(OH) D3 affect innate immunity mechanisms of tuberculosis, but not about the acquired immunity, In-depth study should be investigated Effect of vitamin D on the immune modulation,the dendritic cells,during tuberculosis infection which play a vital function.From some vivo studies we know that dendritic cell phagocytosis of Mycobacterium tuberculosis, which significantly reduced phagocytosis, costimulatory molecules when TB infected,which play a vital function in the immune response of tuberculosis. Therefore, acquired immunity in tuberculosis, dendritic cells play a vital function.Although the affect of 25(OH) D3 in dendritic cell maturation has been widely reported, but the effects of 25(OH)D3 on maturation and Antigen-Presenting function of dendritic cells in tuberculosis It has not been deeply studiedthe main purpose of the study is to investigate the capability of DC,From the marrow-derived Dendritic cells of mice, after stimulation with mycobacterium tuberculosis in the presence or absence of vitamin D.Methods:1. The establishment of vitamin D deficiency, vitamin D and vitamin D adequacy normal three groups of mouse models, ELISA method detected three groups of mouse serum25(OH) D 3 difference..2.Induction and differentiation of bone marrow derived dendritic cells in mice: scanning electron microscopy, light microscopy, cell morphology, cell flow measurement of MHC-II, CD11 C, CD86, CD80 expression.3.The expression of CD80, CD11 C, CD86 and MHC-II in the three groups of mice bone marrow-derived dendritic cells were detected by different conditions.4. To measure the effect of mixed reaction on T lymphocyte proliferation in three groups of mice and CD4+T/CD8+T, CD4+T cells, CD8+T cells, respectively.Results:1.The levels of 25(OH) D3 in the vitamin deficiency group was significantly less than the normal group.2. Differentiation of DC cells in mouse bone marrow cells, Typical dendritic processes can be observed under the microscope,and were detected by cell flow cytometry. The positive rate of DCs cells was high.3. expression of CD86 in the control group was higher than that in 25(OH) D3 group(p<0.05); The mean fluorescence intensity of MHC-II in control group was greater than that of 25(OH) D3 group(p<0.05)4. The lack of group of dendritic cells was increased the level of T cell proliferation, the distributions of CD4 + T cells and CD8 + T cells were detected.CD4 + T in the control group, 25(OH) D3 and the lack of group were 13.12 ± 0.59 and 11.50 ± 0.55、17.02 ± 0.52;The ratio of CD4+T/CD8+T in 25(OH) D3 group was higher than that in control group, The deficiency group was lower than 25(OH) D3 group.Conclusion:1.Vitamin D deficiency mice model was successfully established by reducing the light and dietary intake of vitamin D.2.25(OH) D3 by stimulating the expression of MHC-II and CD86 in the dendritic cells regulated the maturation and function to affected the immune response in tuberculosis...