Study Of Treatment Of Collagen-Induced Arthritis Rats Model With Avastin

ObjectiveTake wistar rats for the establishment of Collagen-Induced Arthritis (CIA)model. After the model was successfully constructed, the rheumatoid arthritis treatment efficacy of different. Avastin concentrations were observed from IL-1β、IL-6、TNF-α and other indicators. Evaluate the effect of Avastin treatment for RA.MethodCollagen Type Ⅱ and Incomplete Freund’s adjuvant are mixing emulsified with ratio of 1:1 (volume to volume). Injected to Wistar rats for first immunization, after 10 days injected for booster immunization. Divided the rats into four groups based on clinical scores (four groups have almost same scores and 10 rats each group):three groups of intra-articular injection of different concentrations (2g/L、4g/L and 8g/L) of Avastin 100μl, continuous administration of one week, the other group in the same manner injection 100μl of saline as a control. Blood collected at different times (0、7 and 20 days) after Avastin treated, detecting IL-1β, IL-6 and TNF-α inflammatory cytokines by enzyme-linked immunosorbent assay; Histological examination:the use of hematoxylin-eosin (HE) staining after the treatment of animal tissue inflammation and joint morphological changes. Tartrate-resistant acid phosphatase staining (TRAP) staining was observed of the incidence of joints osteoclast situation and pannus formation and Immunohistochemical staining observed of vascular endothelial growth factor expression and other pathological conditions.ResultsAfter the second immunization, the arthritis onset in 10-14 days, Model group rats paw swelling is very obvious, There’s statistically significant difference the model group and the normal animals group in dinical scores (P<0.05). After treatment IL-1β, IL-6 and TNF-a cytokines of Avastin the 4g/L concentration treated group observed significantly reduced compared to control group. HE staining found 4g/L concentration Avastin treated animals intra-articular inflammatory cells was significantly lower than the control group. TRAP and immunohistochemical staining was observed in treated animals joint tissue osteoclast formation and vascular endothelial growth factor expression was significantly lower than control group. The statistical analysis was significant difference of 4g/L concentration of drug treatment and control groupsConclusionLate in the pathological that pannus, generating a large number of blood vessels is an important pathological process of arthritis. After Avastin treated that osteoclasts and inflammatory cells decreased significantly, pannus formation and vascular endothelial growth factor expression was significantly inhibited of 4g/L concentration. Avastin has the effect of a potential treatment of rheumatoid arthritis. The study analyzed and discussed hoping to help to find new treatments drug of rheumatoid arthritis.