The Study Of The Preparation Of Ixeris Chinensis （Thumb.） Nakai Total Flavonoids Extract And The Effect And Mechanism To T₂DM

Objectives:To establish the optimal preparation method and quality standard of Ixeris Chinensis (Thumb.) Nakai Total Flavonoids Extract, reveal the effect and mechanism of it to T2DM.Methods:Based on the single factor experiment and orthogonal test, find the optimal preparation method. Establish the quality standard of Ixeris Chinensis (Thumb.) Nakai Total Flavonoids Extract via TLC qualitative identification and HPLC, UV quantitative detection, examing the water and ash content of it according to the pharmacopoeia, establishing the specific chromatogram; Fed the rats with high fat high sugar fodder and then injected the rats with STZ abdominally. Judge the models by the FBG>7.0mmolL-1 and PBG (30min)>11.0mmol·L-1. Fed the model rats with the extracts and XIAOKE and Melbine with 5 weeks, then test the GLU, INS, IR, GHb and blood fat in serum, take the livers and kidneys off the rats,and test the TGF-β,GLUT-4 mRNA via PCR and PPAR-a, PPAR-y protein expression quantity via Western Blot to study the effect and mechanism.Results:The optimal preparation method was ethanol refluxing and HPD100 macroporous resin. And the condition was 40% ethanol,3h-extraction time,1:8-solid-liquid ratio with 3 times. Established the quality standard, the total flavonoids quantity can’t be less than 255.98mg-g-1 and the galuteolin quantity can’t be less than 58.09mg-g-’. Marked 9 mutual peaks as the feature peaks in its specific chromatogram. After feeding the T2DM rats drugs with 5 weeks, the IH group rats compared with the DM group rats, FBG, PBG (P<0.05), INS, IR GHb and TGF-β mRNA expression quantity apparently decreased (P<0.01), and the GLUT-4 mRNA, PPAR-a, PPAR-y protein expression quantity apparently increased (P<0.01).Conclusion:The preparation method of Ixeris Chinensis (Thumb.) Nakai Total Flavonoids Extract is feasible and reasonable. The method of quality standard is accurate, scientific and convenient. The Ixeris Chinensis (Thumb.) Nakai Total Flavonoids Extract can treat the T2DM rats, decrease the blood glucose obviously, adjust the insulin and Ghb, and relieve "a little more than three disease". Besides, it can prevent the DN from the certain degree. The mechanism of it may result from the up adjustment of PPAR-a, PPAR-y protein expression quantity, and down adjustment of TGF-β mRNA and increase the GLUT-4 mRNA, relieving the inflammation and promoting the use of glucose in peripheral tissue, adjust the lipid metabolism and improvement of the sensibility to insulin. The article could provide the deeper research in its preparation and clinic use with good foundation.