Effect Of Electroacupuncture On The Expression Of BDNF And CREB In Rats Cerebral Ischemic Area

Objective:" LI 10" and "SJ 5" within the cerebral cortex of rats with ischemic region surrounding brain-derived neurotrophic factor (BDNF) and cyclic adenosine monophosphate response element binding protein was observed by electrical stimulation (CREB) expression, as well as upper limb motor function of focal cerebral infarction rats behavioral assessment, to explore the mechanism of electric acupuncture treatment of cerebral infarction.Methods:Healthy adult SPF 60 male SD rats, were randomly divided into blank group,sham group, model group and electroacupuncture group, n= 15. Application line plug prepared rat cerebral infarction model of middle cerebralartery occlusion (MCAO) model.EA group received electroacu-puncture treatment. Acupoints:"LI 10" and "SJ 5". Continuous wave,50 Hz, 20 minutes, Once a day. The control group, sham group and model group, only to be bundled fixed at the same time when EA treatment group, not to be treated with electroacupuncture. Using screen test scoring method assessed each group of upper limb nerve injury when the rats in 1,3,7,14,21 days. Each group of rats after treatment, immunohistochemical detect surrounding cortex of rats ischemic area the positive expression of BDNF, CREB and pCREB.Results:1.Each time point net assessment test scores between the ratsThe control group, sham-operated network assessment test score of 0, Id after the highest score, lowest score after 21d, after each time point, the model group and the control group, the differences were statistically significant (P<0.01). With the extension of cerebral ischemia time, the score of model group decreased, indicating that after focal cerebral ischemia there is a certain self-healing. 1d after surgery, EA group compared with the model group, the difference was not statistically significant (P>0.05).3,7d after the EA group compared with the control group, the difference was significant (P<0.01).EA group compared with the model group, the difference was significant, statistically significant (P<0.05).14 and 21d after the EA group compared with model group, the difference was significant, statistically significant (P<0.05), EA group and control group comparison, the difference was not statistically significant (P>0.05).2.Electroacupuncture on MCAO rats cortex BDNF around positive expression in ischemic areaBrain BDNF expression level decreased significantly after modeling, BDNF expression increased after electroacupuncture treatment. The control group and the sham group was not statistically significant, was not statistically significant (P>0.05); model group, EA group and the control group, the difference was significant, statistically significant (P<0.01); EA and model group, the difference was significant (P<0.01).3.Electroacupuncture on MCAO rats ischemic cortex around pCREB, CREB positive expression After modeling the brain CREB, pCREB expression level was significantly decreased after electroacupuncture treatment CREB, pCREB increased expression. The control group and the sham group comparison, the difference was not statistically significant, was not statistically significant (P>0.05); EA group, model group and control group comparison, the difference was significant, statistically significant (P<0.01); EA group comparison with the model group, there was statistically significant (P <0.01).Conclusion:1. Electroacupuncture can improve movement dysfunction after cerebral infarction, and raised the expression of BDNF,CREB and pCREB.2. Electroacupuncture stimulation "LI 10" and "SJ 5" can significantly reduce neural functional defect in rats with cerebral infarction, promote recovery of upper limb motor function.3. Electroacupuncture has the nerve protective effect on cerebral infarction and its mechanism may rather than increase blood flow of BDNF and CREB positive expression level.