The Experimental Study And Exploration Of The Protective Effert Of Cisapride On The Intestinal Barrier Function Under The Condition Of Exsanguine Shock

Objective:This study is mainly to explore the role of intestinal motility drugs in improving the intestinal mucosal barrier function after trauma, and further explore the feasibility of the improvement of intestinal motility and intestinal function failure.Method : Project by hemorrhagic shock model of rats and on gastrointestinal motility drug cisapride on hemorrhagic shock in rats with intestinal peristalsis rate, organ blood flow function, intestinal barrier function and ischemia and perfusion effects. The healthy male Wistar rats, weighed 230 + 20 g, rats(n = 6), blood loss shock model with modified Wiggers method, divided into normal control group, hemorrhagic shock group, cisapride(Shanghai source Pharmaceutical Co., Ltd. production) treatment group, the distilled water control group, except the normal and shock control group, the other two groups were given two times blood volume fluid resuscitation were oral cisapride and distilled water. Observed experimental drug cisapride treatment of hemorrhagic shock rats survival rate, blood pressure, organ blood flow, intestinal creep rate, bacterial translocation, intestinal tissue of Dao and D-lactate in situation.Result:1 prokinetic drug cisapride and improve normal intestinal peristalsis shock rats rate.The results showed that the intestinal peristalsis rate decreased after hemorrhagic shock in rats, which was 12% in the normal group, and the normal peristalsis function of the intestinal tract decreased significantly(p<0.01). The normal animal after administration of cisapride in the treatment group, the creep rate was significantly higher than the normal group(p<0.05). Shock animal after administration, compared with the control group shock, cisapride treatment group can significantly improve the intestinal peristalsis function(p<0.01).2 prokinetic drug cisapride can shock rats intestinal mucosa microcirculation blood flow improved significantly.To drug treatment after 0.5h, compared with shock group, recovery group and distilled water group, the intestinal mucosa microcirculation blood flow increased significantly(p<0.01), cisapride treatment group blood flow increased significantly; to drug treatment for 2.5 hours, compared with the distilled water group, cisapride therapy group rat small intestinal mucosa microcirculation blood flow has a significant difference(p<0.01).3 prokinetic drug cisapride treatment improve the intestinal barrier function of septic shock rats, significantly decreased DAO, D-levels of plasma lactic acid.3.1 organ bacterial translocation. Cisapride group in plasma and liver significantly reduced the number of colonies and cisapride in the treatment group than the shock group of bacteria falling number decreased significantly(p<0.01).3.2 plasma DAO content. Two amine oxidase(DAO) activity is an indicator of intestinal permeability of intestinal dysfunction. Experimental rats with hemorrhagic shock after plasma Dao levels increased significantly(p<0.01), cisapride group plasma Dao levels were significantly lower than pure shock group and distilled water group(p<0.01).3.3 plasma D-lactic acid content. D-lactic acid content is a reflection of intestinal dysfunction of the intestinal mucosal damage detection index. Compared with the rats in the normal group, blood loss shock rat plasma D-lactate level increased significantly(p<0.01); compared with the shock group and distilled water group and cisapride group D-lactic acid content decreased significantly(p<0.01).4 prokinetic drug cisapride treatment can significantly improve the 24 hours of hemorrhagic shock rats survival rate.Simple physiological saline liquid resuscitation 24 h survival rate for 52.37%, resuscitation with normal saline plus cisapride treatment 24 h survival rate was 87.98%, and simple saline fluid resuscitation group compared with significant differences(P< 0.05).Conclusion: This study verify the protective effect of prokinetic drug cisapride on intestinal barrier function injury, for future intestinal motility drugs can improve the intestinal barrier function provides an experimental basis for theoretical basis and further research, so as to provide a new therapeutic approach for the treatment of intestinal dysfunction.