Research And Application Of The Integrated Testing Method Of Opioid Drugs In Human Body

Objective: to abiotic suspected opioid poison and opioid dependence or occasionally drug users of the biological sample(urine, blood,), set up the sample pretreatment method,using GC-MS or GC analysis.Methods: to establish a team with high sensitivity, fast analysis speed, accurate, and measuring method of less dosage of examination etc.Methods: using colloidal gold method speed screening suspicious. Tested by GC and GC-MS, the mass-to-charge ratio retention time, or ions to qualitatively identify suspicious target; Within a standard method for quantitative experiment, computing suspicious target percentage. In biological samples(blood, urine) before processing, by screening method(colloidal gold), by solid phase extraction(SPE) method of extraction, derivatization reagent reaction, by GC/MS method for full scan or select a specific ion mass ratio(m/z)statutory charge confirmation, within standard method(internal standard for SKF525 a)for quantitative analysis. Results: the heroin, morphine and codeine in biological samples and the other under the condition of GC detection limit(LOD) for 2 μg/ml, quantitative limits(LOQ) for 20 μg/ml. Provided by the ministry of public security material evidence identification of suspicious drugs through the established method, check out the heroin and 6- acetyl codeine, the heroin content is 38.4%, the final result, robust and Z score less than or equal to 2, ability to verify through with satisfied results. Confirm the feasibility of this test method that blind detection and accuracy. By the blood and urine, respectively, for standard working curve, the linear range of 25-400 ng/ml, GC-MS detection limit(LOD)for 5 ng/ml, quantitative limit(LOQ) for 25 ng/ml. Abuse of heroin addicts biological sample(urine, blood), and through the established method, codeine, which was detected in the urine morphine concentration is 60.14 ng/mL, 55.99 ng/mL, 47.24 ng/mL single acetyl morphine, detection of morphine content in blood of 82.40 ng/mL, 36.61 ng/mL codeine content; The illegal facts confirmed drug heroin Through quantitative analysis of the feasibility and accuracy of this test method is validated. Conclusion: this method issimple, rapid, high sensitivity, accurate quantitative and qualitative applicable to the drug or drug abusers blood, the urine screening and the confirmation test.