Canine Alveolar Bone--derived Stem Cells And Bone Marrow Mesenchymal Stromal Cells :A Comparative Study Of The Osteogenic Potential

Objective: To compare the osteogenic ability of two different canine stem cells in the application of craniomaxillofacial bone tissue engineering in vitro, namely alveolar bone-derived stem cells(ABSC) and bone marrow mesenchymal stromal cells( BMSC).Methods: ABSC and BMSC were prepared from the alveolar bone and bone marrow of a same dog. The third generation of these cells were used for the following studies.Firstly, the two cells was checked by MTT assay about their proliferative ability.Secondly, after osteogenic induction, the osteogenic differentiation ability was detected by alizarin red staining and alkaline phosphatase staining. Thirdly, after osteogenic induction for 7, 14 and 21 d, the activity of intracellular alkaline phosphatase was detected by the p NPP method. Fourthly,after osteogenic induction,the expression of COL-1, BMP-2, ALP, Runx2 and OCN was analyzed by RT-PCR method.Result: The proliferative ability of ABSC was higher in both of them. After osteogenic induction,ABSC formed more calcium nodules and alkaline phosphatase than BMSC.The results showed that the expression of COL-1 in ABSC was higher than that in BMSC and both of them decreased gradually. BMP-2 was higher in ABSC early and in BMSC finally, both of them showed an upward trend. The expression of ALP during them reached the highest point at 14 d. Runx 2 in ABSC reached the peak at 14 d and the expression of BMSC in Runx2 rised with time. OCN was significantly expressed in ABSC at 14 and 21 d,and was higher in BMSC.Conclusion: The osteogenic abilities of ABSC were similar with that of BMSC,which is an ideal seed cell in the research of bone tissue engineering.