Experimental Study On Toxicity Of Benzene

Objective: BALB/c mice and Wistar rats as experimental object, to study the role of benzene toxicity to animals and their mechanism, in order to further study the reasonable use of benzene and benzene pollution prevention and control provides some references for the impact on human health.Methods: The BALB/c mice and the Wistar rats were administered with different concentration of benzene for 21 days, by automatic biochemical analyzer test the effects of benzene on blood composition, enzyme linked immunosorbent assay（enzyme- linked immunosorbent assay, ELISA） to detect the benzene to cyclic adenosine monophosphate（c AMP）, cyclic guanosine phosphate c GMP) and heat shock protein-70（HSP-70）, Luteinizing hormone（LH）, follicle-stimulating hormone（FSH）, estradiol（E₂）, prolactin（PRL） levels. Results: 1. When BALB/c mice were administered with benzene at the doses of 0.12, 0.3, 1.2 g/kg body weight respectively for 21 days, groups of animals in the blood, globulin, albumin, phosphorus, calcium ions, white blood cells and platelets levels than the control group were reduced by 22.51%, 37.99% and 56.69%, 13.41%, 26.81% and 33.29%, 4.48%, 14.46% and 22.07%, 24.28%, 63.64% and 86.26%, 39.90%, 52.04% and 61.80%, 23.80%, 27.36% and 32.15%,respectively; c AMP level than the control group was reduced by 8.19%, 41.11% and 8.19% respectively; Potassium and 0.3, 1.2 g/kg body weight groups of magnesium ions were increased by 12.88%, 34.52%, 57.32% and 12.88%, 91.20%,respectively; c GMP level than the control group was increased by 21.60%, 21.60% and 21.60%, respectively; HSP-70 level than the control group was increased by 24.16%, 24.16% and 24.16%, respectively; c AMP level in liver, kidney tissues than the control group was reduced by 19.39%, 41.61% and 61.79%,25.00%, 40.05%, and 176.13%,respectively;c GMP level was reduced by 8.25%, 13.11% and 79.63%, 33.43%, 78.19% and 88.10%, respectively; HSP-70 level in liver tissue than the control group was increased by 17.44%, 17.44% and 17.44% respectively; In the kidney tissues was reduced by 4.94%, 8.41% and 4.94%,respectively.2. When Wistar rats were treated with benzene at the doses of 0.19, 0.38, 0.76 g/kg body weight for 21 days, total bilirubin in animal plasma, aspertate aminotransferase, creatinine, creatine kinase and 0.38, 0.76 g/kg body weight groups of urea levels than the control group were increased by 134.40%, 134.40% and 173.63%,70.76%, 85.44% and 106.61%, 24.54%, 67.46% and 84.50%, 151.35%, 180.85% and 245.54%,180.85%, 245.54%,respectively; c AMP,c GMP and HSP-70 levels were increased by 41.84%, 264.02% and 314.23%, 31.29%, 40.46% and 272.14%,40.46%, 187.37% and 1484.21%, respectively.LH, E₂, FSH, PRL levels than the control group were enhanced by 218.55%, 223.39% and 375.40%, 80.57%, 101.52% and 471.43%, 516.16%, 702.62% and 1169.89%, 117.49%, 213.49% and 237.47% respectively; amylase level was reduced by 62.03%, 63.66% and 62.03%,respectively; c AMP, HSP-70 levels in rat cerebellum than the control group were enhanced by 48.05%, 93.50% and 176.62%, 0.02%, 40.74% and 99.07% respectively; c GMP level than the control group was reduced by 39.85%, 48.66% and 39.85%,respectively; c AMP,c GMP levels in the thalamus of rats, 0.19, 0.38 g/kg body weight groups than the control group were reduced by 52.69%,50.12%, 80.38%, 71.41%,respectively; HSP-70 level than the control group was increased by 34.78%, 34.78% and 34.78% respectively.E₂, FSH, PRL levels in the rat brain cortex than the control group were increased by 98.43%, 98.43% and 141.36%, 3.88%, 31.96% and 72.73%, 2.49%, 41.29% and 2.49%,respectively; E₂, FSH, PRL levels In the hippocampus of rats than the control group were enhanced by 100.51%, 193.23% and 204.82%, 46.39%, 62.89% and 96.07, 3.73%, 22.47% and 84.18%.Conclusion Benzene can cause liver, kidney and pancreas injury, plasma protein decreased, detoxification function, immune function, and plasma colloid osmotic pressure is reduced, the ratio of cyclic adenosine monophosphate（c AMP） and Cyclic guanosine phosphate（c GMP） levels changes, causing metabolic disorder, cause the body damage or lesions; benzene can stimulate the pituitary gland secretion of follicle stimulating hormone（FSH） and luteinizing hormone（LH）, accelerate the synthesis and secretion of estradiol（E₂）, cause female precocious puberty, menstrual cycle disorder, gonad hypofunction, Men feminine and other symptoms.