Studies On Regulation Of Lipid Metabolism And Mechanism Of Effective Ingerdients From Valeriana Jatamansi Jones

Objective Studies on regulation of lipid metabolism and mechanism of effective ingrediensts from valerinan jatamansi jones.Method 1.MTT was used to detect different concentrations of liquid on the survival of HepG2 cells, determined2mmol/L oleic acid induced by human hepatoma HepG2 steatosis model,160μg/mL fenofibrate as a positive control, Oil Red O staining and enzyme were detected the deposition of lipid droplets and triglyceride content in HepG2 cells with 6,12.5, 25 μg/mL Valjatrate E. Western Blot method was detectedthe protein expression of ApoA5 in HepG2 cells.2. The high fat diet to establish animal models of hyperlipidemia rats, 2mg/kg/d of simvastatin as a positive control, fed 7.5mg/kg/d,15mg/kg/d,30mg/kg/d iridoids of valerinan jatamansi jones. Double reagent was measured the content of TC, TG, LDL-C, HDL-C in serum and liver tissue of rats; Micro-plate wasmeasured the content of TBA, ALT, ASTinserum; Eosin (HE) staining was observed the pathological changes of liver tissue; Colorimetric wasdetected rat liver tissue in LPL, HL activity; ELISA was used to detect protein expression of apolipoprotein ofApoA5, PPAR-a, SREBP-1c, LXR-α.Results 1.iridoids of valerinan jatamansi jones can inhibit lipid accumulation in HepG2 cells induced by oleic acid, and its mechanism may be related to increase protein expression ApoA5.2. Hyperlipidemia rat animal model successfully established, compared with the model group,7.5mg/kg/d,15mg/kg/d,30mg/kg/d iridoidsof valerinan jatamansi jones group can slowing weight of rats in different degrees; reduced content of TC, TG, LDL, and increased content of HDL inserum and liver tissue, reducing the TG content was the most obvious (P<0.01); low dose group can significantly reduce rat liver index (P<0.05),high dose group reduce TBA, ALT, AST content of in serum(P<0.01);Liver tissue pathological observation showed that, Low-dose group compared with the control group showed no vacuoles incytoplasmic and no lesions, havinghepatoprotective effect;Further studies showed that the mechanism indicated lowand high dose group ofiridoids of valerinan jatamansi jonescan enhance activity ofLPL(P<0.01)and HL(P<0.05);high dose group can increase ApoA5, PPAR-ain protein expression(P<0.01), lowand high dose groupreduce SREBP-1c, LXR-a in protein expression(P<0.01).Conclusion 1.Valjatrate Ecan inhibit lipid accumulation in HepG2 cells induced by oleic acid, and its mechanism may be related to increase protein expression ApoA5.2. iridoids of valerinan jatamansi jones could promote blood lipid, liver lipid metabolism, and has a hepatoprotective effect, the mechanism of protein expression of PPAR-a may be related to activation, inhibition of SREBP-lc, LXR-a protein expression, and thereby regulate the expression ApoA5 improve lipid metabolism-related enzyme LPL, HL’s activity. Described above, jatamansi active ingredient has a good role in the regulation of lipid metabolism.