Effects Of TSG-6 On The Proliferation And Apoptosis Of Human Pathological Scar Fibroblasts And Its Mechanism

Background and Objective The skin repair method is divided into two types.One way of skin repair which can realize the skin structural integrity and the function recovery through simple epithelization only involves the epidermis. Another is the skin damage repair which is involved in the dermis and subcutaneous tissue, This approach to repair skin will cause the excessive skin healing and form the pathological scar. Hyperplastic tissue of pathological scar which is higher than the surrounding normal skin not only affect the appearance, but also produce some symptoms, such as itching,pain and even scar contracture. Scar contracture in the joints often results in severe joint dysfunction, bringing great pain to the patients. Therefore,the prevention and treatment of skin scar formation after trauma is an important problem in the field of wound healing. TSG-6 is a kind of participation in a variety of inflammatory reaction of anti-inflammatory factor found recently, its expression may be an important factor leading to the formation of pathological scar. The purpose of this study is to explore the effects of restructure TSG-6 on proliferation and apoptosis of pathological scar fibroblasts; to study the TSG-6 on the role of pathological scar fibroblasts proliferation and apoptosis; and further to gain the new ideas and methods in the clinical treatment and prevention of pathological scar.Methods The pathological scar samples of 6 cases(male 2 cases, female4 cases), from The First Affiliated Hopital of Anhui Medical University orthopedic ward and outpatient surgery resection of pathological scar tissue, age of the patients ranged from 4 to 27 years, an average of 16.8years, belong to the first treatment, not by other treatment and pathological scar prominent symptoms, duration of 6 to 12 months,and then were cultured to pathological scar fibroblasts with adherence method.Cells used in this study were propagated for 3-4 times before use. The pathological scar fibroblasts were cultured with 2 μg/ml of recombinant human TSG-6 protein for 48 h. The effects of TSG-6 on cell proliferation were then assessed by immunohistochemical method at the indicate time. The rate of apoptosis induced by TSG-6 was determined by flow cytometry.Western blot was performed to detect the expression level of caspase-3 and p53 after TSG-6 treated.Results After treated with TSG-6 for 48 h,cells expressing proliferating cell nuclear antigen,an indicator of cell proliferation, reduced significantly(P<0.05). On the other hand, the rate of apoptosis of pathological scar fibroblasts induced by TSG-6 with 2 μg/ml was increased(P<0.05). The expression levels of apoptosis related proteins caspase-3and p53 were significantly increased in TSG-6 treated group.Conclusions There is a close relationship between TSG-6 and the occurrence of human pathological scar. TSG-6 may inhibit proliferation and induce apoptosis of human pathological scar fibroblast in vitro. The inhibition of proliferation might be caused by inhibition of PCNA gene expression,and the induction of apoptosis might be associated with promotionexpression of caspase3 and p53.