Changes And Clinical Significance Of Interleukin-17、Interleukin-35 And T Lymphocyte Subsets In The Peripheral Blood Of Children With Acute Lymphoblastic Leukemia

ObjectiveTo explore the expression of T lymphocyte subsets,and detect the levels of IL-17 and IL-35 in the peripheral blood of children with acute lymphoblastic leukemia.In this paper, we preliminarily discussed about the significance of immune factors in children with acute lymphoblastic leukemia.MethodsWe collected one hundred and forty-one cases of children with ALL from Jan. 2014 to Apr. 2015 in the Third Affiliated Hospital of Zhengzhou University and the First Affiliated Hospital of Zhengzhou University.They were divided into two groups: preliminary diagnosis group(n=76) who were confirmedly diagnosed but not yet treated, remission group(n=65) who were confirmedly diagnosed and treated systematically with chemotherapy;and 45 healthy children were selected as the control group.We used flow cytometry and ELISA to detect the T lymphocyte subsets and the corresponding cytokines. The data were processed and analyzed by statistical software SPSS 17.0.Results1.The results of IL-17The IL-17 concentrations of preliminary diagnosis group and remission group were significantly lower than that of control group(P<0.05),and the level of preliminary diagnosis group was significantly lower than that of remission group(P<0.05).2.The results of IL-35The IL-35 concentrations of preliminary diagnosis group and remission group were significantly higher than that of control group(P<0.05),and the level of preliminary diagnosis group was significantly higher than that of remission group(P<0.05).3.The results of T lymphocyte subsetsThe expressions of CD3+ T and CD4+ T cells in preliminary diagnosis group and remission group were significantly lower than that of control group(P<0.05),and the CD8+ T cells level increased significantly(P<0.05),significant difference was found between preliminary diagnosis group and remission group(P<0.05).4.The correlation analysisThe expression of CD4+ T cells of ALL children was positively correlated with the level of IL-17(r=0.678,P<0.001),but negtively correlated with the level of IL-35(r=-0.688,P<0.001).ConclusionsThe occurrence of children with acute lymphoblastic leukemia may be associated with cellular immune dysfunction,and the concentrations of IL-17 and IL-35 may also paly an important role in its pathogenesis.