Studies On The Non-alkaloids Of The Radix Of Aconitum Vilmorinianum Komarov And Their Diversification After Processing

In this paper, a research on the non-alkaloid constituents of Aconitum vilmorinianum Komarov and their contents of changements after processing had been carried out. Phytochemical investigation on the non-alkaloids of chloroform extraction parts from the methanol extract of A. vilmorianum led to the isolation of 18 compounds. At present, the structures of ten compounds were determined by spectroscopic analysis(MS, 1D and 2D NMR). They were identified to be(3R,5S,8R,9R,10 S,14R)-3,17-dihydroxycleistantha-12,15-diene(1), 16,17–epoxy-3,15-dihydroxycleistantha-12-ene(2), Methyl-3-Hydroxy-4-(Benzoyl- amino) Benzoate(3), Phenanthridin-6(5H)-one(4), carbazole(5), p-Aminocinnamic acid methyl ester(6), isotalatizidine(7), chasmaconi tine(8), 1,3-dipalmitin(9) and β-sitosterol(10), in which, 1, 2 and 3 were new compounds.The radix of A. vilmorinianum has two processed methods: boiling for the fresh material, while both boiling and steaming for the dry material. Based on the separation of four non-alkaloids(compounds 1-4), a high-performance liquid chromatography was established for the comparation of the difference of their contents between the raw materials and the processed products. The results revealed that compound 1 only existed in dry medicinal material, and it can be speculated that compound 1 derivated from compound 2 in the drying process, and then it thoroughly transformated to other components during the boiling and steaming process.Compound 2 exited in both fresh and dry materials, it disappeared after boiling and its content reduced after boiling and steaming. It suggested that compound 2 transformed during procession as well. The content of compound 3 was increased in both processed methods; it may be owing to the addition of the fruits of Gleditsia sinensis in processing. Because we found compound 3 existed in this auxiliary material by HPLC analysis. The content of compound 4 was decreased after both processed methods, which can be infered that compound 4 had changed in processing.In the end, we summarized the research progress of non-alkaloids of Aconitumand cleistanthane diterpenoids by consulting the literatures.