Study Of Cold Plasma Radiation Effects On C57BL/6 Laboratory Mice With Malignant Melanoma

Objective: By constructing a research model of C57BL/6 laboratory mice with malignant melanoma, to observe the cold plasma’s radiation effects on solid tumour, trying to draw its dose – effect relationship and evaluating the possibility of cold plasma therapy as a part of cancer treatment.Method: 6-week-old C57BL/6 mice are chosen to be the research object, 5 in per group, constructing melanoma animal testing model by injecting B16F10 tumour cell. Using different conditions of cold plasma to deal with those grown tumour masses, recording the change of mass volume and comparing with control group.Results: 1. After plasma therapy, the average mass volume of experimental group is much smaller than control group, the difference between those two groups shows significant differences. 2. There seems to be a negative correlation between radiating time(dose) and the mass volume after ridiating cold plasma. Those masses need more time doubling there volume compared with those control group and groups taking less radiation.Conclusion: 1. The cold plasma therapy shows significant treatment effect on those tumour mass by causing programmed cell death(PCD, or apoptosis) and doing growth inhibition to tumour cell. 2. The ability of causing apoptosis and doing growth inhibition has a positive correlation with radiating time(dose). 3. There seems to be a possibility of cold plasma therapy as a part of cancer treatment.